| Ethanol stimulates bile acid formation in primary human hepatocytes. | |
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MedLine Citation:
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PMID: 17976534 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The conversion of cholesterol to bile acids is a key pathway for elimination of cholesterol from the body, thereby reducing the risk of arteriosclerosis. Moderate consumption of ethanol has been shown to have preventive effects on cardiovascular disease and decrease the risk of gallstone formation. In the present study primary human hepatocytes were used to investigate if ethanol affected bile acid synthesis. Hepatocytes were prepared from donor liver (n=11) and treated with ethanol, 7.7 or 50 mM, for 24 h. mRNA levels for enzymes in bile acid synthesis pathways were studied and bile acid synthesis was analyzed. Treatment with 7.7 mM ethanol increased cholic acid synthesis by 20% and treatment with 50 mM ethanol up-regulated cholic acid formation by 60%. The synthesis of cholic acid increased more than that of chenodeoxycholic acid, indicating that the classical pathway for bile acid synthesis was up-regulated. Increased bile acid levels in the cells treated with ethanol were seen after approximately 20 h. mRNA expression of CYP7A1, CYP27A1, and CYP8B1 in the hepatocytes was not affected by alcohol exposure. |
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Authors:
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Lisa-Mari Nilsson; Jan Sjövall; Stephen Strom; Karl Bodin; Greg Nowak; Curt Einarsson; Ewa Ellis |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-10-15 |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 364 ISSN: 1090-2104 ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2007 Dec |
Date Detail:
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Created Date: 2007-11-20 Completed Date: 2007-12-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 743-7 Citation Subset: IM |
Affiliation:
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Division of Gastroenterology and Hepatology, Department of Medicine, Karolinska Institute, Karolinska University Hospital Huddinge, S-14186 Stockholm, Sweden. lisa-mari.nilsson@ki.se |
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| MeSH Terms | |
Descriptor/Qualifier:
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Bile Acids and Salts
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biosynthesis* Cells, Cultured Dose-Response Relationship, Drug Ethanol / administration & dosage* Hepatocytes / drug effects, metabolism* Humans Up-Regulation / drug effects |
| Chemical | |
Reg. No./Substance:
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0/Bile Acids and Salts; 64-17-5/Ethanol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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