| Ethanol production from xylose with the yeast Pichia stipitis and simultaneous product recovery by gas stripping using a gas-lift loop fermentor with attached side-arm (GLSA). | |
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MedLine Citation:
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PMID: 10620264 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The bioconversion of xylose into ethanol with the yeast Pichia stipitis CBS 5773 is inhibited when 20 g/L of ethanol are present in the fermentation broth. In order to avoid this limitation, the fermentation was carried out with simultaneous recovery of product by CO(2) stripping. The fermentation was also improved by attaching a side-arm to the main body of a classical gas-lift loop fermentor. This side-arm increases the liquid circulation, mass transfer, and gas distribution, reducing the amount of oxygen in the inlet gas necessary to perform the fermentation of xylose under microaerobic conditions (K(L)a approximately 16 h(-1)). The continuous stripping of ethanol from the fermentation broth in this new bioreactor system allowed the consumption of higher xylose concentrations than using Erlenmeyer shaker flasks, improved significantly the process productivity and provided a clean ethanol solution by using an ice-cooled condenser system. Finally, a fed-batch fermentation was carried out with a K(L)a = 15.8 h(-1). Starting with 248.2 g of xylose, 237.6 g of xylose was consumed to produce 88.1 g of ethanol which represents 72.6% of the theoretical yield (47.2 g/L of ethanol was recovered in the condenser, while 9.6 g/L remained in the fermentation broth). |
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Authors:
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J M Domínguez; N Cao; C S Gong; G T Tsao |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Biotechnology and bioengineering Volume: 67 ISSN: 0006-3592 ISO Abbreviation: Biotechnol. Bioeng. Publication Date: 2000 Feb |
Date Detail:
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Created Date: 2000-02-15 Completed Date: 2000-02-15 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7502021 Medline TA: Biotechnol Bioeng Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 336-43 Citation Subset: IM |
Copyright Information:
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Copyright 2000 John Wiley & Sons, Inc. |
Affiliation:
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Laboratory of Renewable Resources Engineering (LORRE), Purdue University, West Lafayette, Indiana 47907, USA. jmanuel@uvigo.es |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Air Movements Bacteriological Techniques Bioreactors* Biotechnology / instrumentation*, methods Ethanol / isolation & purification, metabolism* Fungal Proteins / metabolism Gases Glass Pichia / growth & development, metabolism* Xylose / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Fungal Proteins; 0/Gases; 0/Xylose; 64-17-5/Ethanol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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