Document Detail

Ethanol production from wheat straw hemicellulose hydrolysate by Pichia stipitis.
MedLine Citation:
PMID:  11267696     Owner:  NLM     Status:  MEDLINE    
Ethanol production was evaluated from wheat straw (WS) hemicellulose acid hydrolysate using an adapted and parent strain of Pichia stipitis. NRRL Y-7124. The treatment by boiling and overliming with Ca(OH)(2) significantly improved the fermentability of the hydrolysate. Ethanol yield (Yp/s) and productivity (Qp av) were increased 2.4+/-0.10 and 5.7+/-0.24 folds, respectively, compared to neutralized hydrolysate. Adaptation of the yeast to the hydrolysate resulted further improvement in yield and productivity. The maximum yield was 0.41+/-0.01 g(p) g(s)(-1), equivalent to 80.4+/-0.55% theoretical conversion efficiency. Acetic acid, furfurals and lignins present in the hydrolysate were inhibitory to microbial growth and ethanol production. The addition of these inhibitory components individually or in various combinations at a concentrations similar to that found in hydrolysate to simulated medium resulted a reduction in ethanol yield (Yp/s) and productivity (Qp av). The hydrolysate used had the following composition (expressed in g x l(-1)): xylose 12.8+/-0.25; glucose 1.7+/-0.3; arabinose 2.6+/-0.21 and acetic acid 2.7+/-0.33.
J N Nigam
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biotechnology     Volume:  87     ISSN:  0168-1656     ISO Abbreviation:  J. Biotechnol.     Publication Date:  2001 Apr 
Date Detail:
Created Date:  2001-03-27     Completed Date:  2001-08-30     Revised Date:  2002-11-01    
Medline Journal Info:
Nlm Unique ID:  8411927     Medline TA:  J Biotechnol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  17-27     Citation Subset:  IM    
Biochemistry Division, Regional Research Laboratory, 785 006, Assam, Jorhat, India.
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MeSH Terms
Acetic Acid / pharmacology
Biotechnology / methods
Culture Media / chemistry
Ethanol / metabolism*
Furaldehyde / pharmacology
Lignin / pharmacology
Pichia / drug effects,  metabolism*
Plant Shoots / chemistry
Polysaccharides / chemistry*,  metabolism
Triticum / chemistry*
Xylose / metabolism
Reg. No./Substance:
0/Culture Media; 0/Polysaccharides; 0/Xylose; 64-17-5/Ethanol; 64-19-7/Acetic Acid; 8024-50-8/hemicellulose; 9005-53-2/Lignin; 98-01-1/Furaldehyde

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