Document Detail


Ethanol inhibits zymosan-stimulated and enhances nonstimulated platelet-activating factor production in a clonal macrophage cell line.
MedLine Citation:
PMID:  2319456     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ethanol was examined for its effects on zymosan phagocytosis and synthesis of platelet-activating factor (PAF) using a clonal macrophage cell line. PAF, identified as 1-O-alkyl-2-acetyl-sn-glycerol-3-phosphocholine, is a biologically active ether phospholipid produced by various cells types. PAF also activates a variety of inflammatory cells including the cells which produce PAF. Ethanol at 20 to 100 mM inhibited phagocytosis of unopsonized zymosan and concomitantly decreased PAF concentration in the stimulated macrophages. In contrast, in cells not stimulated with zymosan, ethanol increased the recoverable PAF. Ethanol, at 100 mM, inhibited the uptake of exogenous PAF but did not alter the distribution of PAF metabolites in the clonal macrophage cell line. The effect ethanol has on PAF synthesis appears dependent on the activation state of the cell. Two situations were identified in this study: 1) one linked to phagocytosis or cell stimulation which is inhibited by ethanol, and 2) PAF synthesis which is not dependent on overt cell activation that is enhanced by ethanol.
Authors:
R C Baker; M Tucker; K L Clay
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of pharmacology and experimental therapeutics     Volume:  252     ISSN:  0022-3565     ISO Abbreviation:  J. Pharmacol. Exp. Ther.     Publication Date:  1990 Mar 
Date Detail:
Created Date:  1990-05-03     Completed Date:  1990-05-03     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0376362     Medline TA:  J Pharmacol Exp Ther     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1028-33     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, University of Colorado Health Sciences Center, Denver.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Ethanol / pharmacology*
Macrophages / drug effects*,  enzymology,  metabolism
Phagocytosis / drug effects*
Platelet Activating Factor / biosynthesis*,  metabolism
Zymosan / antagonists & inhibitors*
Grant Support
ID/Acronym/Agency:
AA07157/AA/NIAAA NIH HHS; HL34303/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Platelet Activating Factor; 64-17-5/Ethanol; 9010-72-4/Zymosan

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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