Document Detail

Ethanol induces peroxynitrite-mediated toxicity through inactivation of NADP+-dependent isocitrate dehydrogenase and superoxide dismutase.
MedLine Citation:
PMID:  18405671     Owner:  NLM     Status:  MEDLINE    
It has been reported that chronic alcohol administration increases peroxynitrite hepatotoxicity by enhancing concomitant production of nitric oxide and superoxide. Several studies have shown the importance of superoxide dismutase (SOD) in protecting cells against ethanol-induced oxidative stress. Recently, we demonstrated that the control of cytosolic and mitochondrial redox balance and the cellular defense against oxidative damage is one of the primary functions of NADP+-dependent isocitrate dehydrogenase (ICDH) through to supply NADPH for antioxidant systems. In this report, we demonstrate that ethanol induces the peroxynitrite-mediated cytotoxicity in HepG2 cells through inactivation of antioxidant enzymes such as ICDH and SOD. Upon exposure to 100mM ethanol for 3days to HepG2 cells, a significant decrease in the viability and activities of ICDH and SOD was observed. The ethanol-induced inactivation of antioxidant enzymes resulted in the cellular oxidative damage and modulation of redox status as well as mitochondrial dysfunction in HepG2 cells. The cytoxicity of ethanol and inactivation of antioxidant enzymes were effectively protected by manganeses(III) tetrakis(N-methyl-2-pyridyl) porphyrin, a manganese SOD mimetic, and N'-monomethyl-l-arginine, a nitric oxide synthase inhibitor. These results indicate that ethanol toxicity is mediated by peroxynitrite and the peroxynitrite-mediated damage to ICDH and SOD may be resulted in the perturbation of the cellular antioxidant defense systems and subsequently lead to a pro-oxidant condition.
Eun Sun Yang; Jin Hyup Lee; Jeen-Woo Park
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-03-19
Journal Detail:
Title:  Biochimie     Volume:  90     ISSN:  0300-9084     ISO Abbreviation:  Biochimie     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-07-29     Completed Date:  2008-10-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1264604     Medline TA:  Biochimie     Country:  France    
Other Details:
Languages:  eng     Pagination:  1316-24     Citation Subset:  IM    
School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University, 1370 Sankyuk-Dong, Taegu 702-701, Korea.
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MeSH Terms
Cell Line, Tumor
Cell Survival / drug effects
Enzyme Activation / drug effects
Ethanol / pharmacology*
Isocitrate Dehydrogenase / metabolism*
Mitochondria / drug effects,  metabolism
NADP / metabolism*
Peroxynitrous Acid / biosynthesis,  toxicity*
Superoxide Dismutase / metabolism*
Reg. No./Substance:
14691-52-2/Peroxynitrous Acid; 53-59-8/NADP; 64-17-5/Ethanol; EC Dehydrogenase; EC Dismutase

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