| Ethanol induces heterotopias in organotypic cultures of rat cerebral cortex. | |
| | |
MedLine Citation:
|
PMID: 15166098 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Abnormalities in the migration of cortical neurons to ectopic sites can be caused by prenatal exposure to ethanol. In extreme cases, cells migrate past the pial surface and form suprapial heterotopias or 'warts'. We used organotypic slice cultures from 17-day-old rat fetuses to examine structural and molecular changes that accompany wart formation. Cultures were exposed to ethanol (0, 200, 400 or 800 mg/dl) and maintained for 2-32 h. Fixed slices were sectioned and immunolabeled with antibodies directed against calretinin, reelin, nestin, GFAP, doublecortin, MAP-2 and NeuN. Ethanol promoted the widespread infiltration of the marginal zone (MZ) with neurons and the focal formation of warts. The appearance of warts is time- and concentration-dependent. Heterotopias comprised migrating neurons and were not detected in control slices. Warts were associated with breaches in the array of Cajal-Retzius cells and with translocation of reelin-immunoexpression from the MZ to the outer limit of the wart. Ethanol also altered the morphology of the radial glia. Thus, damage to the integrity of superficial cortex allows neurons to infiltrate the MZ, and if the pial-subpial glial barrier is also compromised these ectopic neurons can move beyond the normal cerebral limit to form a wart. |
| | |
Authors:
|
Sandra M Mooney; Julie A Siegenthaler; Michael W Miller |
Related Documents
:
|
16172748 - Regulation and function of neurogenesis in the adult vertebrate brain. 3604578 - Cortical dysplasia associated with massive ectopia of neurons and glial cells within th... 20071458 - Chondroitin sulfate acts in concert with semaphorin 3a to guide tangential migration of... 8601808 - Role of neuron-glial junctional domain proteins in the maintenance and termination of n... 12122078 - Neural correlates of structure-from-motion perception in macaque v1 and mt. 11665758 - Building blocks for electronic spiking neural networks. |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. Date: 2004-05-27 |
Journal Detail:
|
Title: Cerebral cortex (New York, N.Y. : 1991) Volume: 14 ISSN: 1047-3211 ISO Abbreviation: Cereb. Cortex Publication Date: 2004 Oct |
Date Detail:
|
Created Date: 2004-09-10 Completed Date: 2004-11-09 Revised Date: 2007-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 9110718 Medline TA: Cereb Cortex Country: United States |
Other Details:
|
Languages: eng Pagination: 1071-80 Citation Subset: IM |
Affiliation:
|
Department of Neuroscience and Physiology, SUNY Upstate Medical University, Syracuse, NY 13210, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cerebral Cortex* Choristoma / chemically induced*, pathology* Dose-Response Relationship, Drug Embryo, Mammalian Ethanol / toxicity* Female Organ Culture Techniques Pregnancy Rats |
| Grant Support | |
ID/Acronym/Agency:
|
AA06916/AA/NIAAA NIH HHS; AA07568/AA/NIAAA NIH HHS |
| Chemical | |
Reg. No./Substance:
|
64-17-5/Ethanol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Human brain regions involved in recognizing environmental sounds.
Next Document: Remembering our past: functional neuroanatomy of recollection of recent and very remote personal eve...