Document Detail

Ethanol exposure decreases glutamate uptake in the nucleus accumbens.
MedLine Citation:
PMID:  15770106     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: An increased level of extracellular glutamate is a key neurochemical feature associated with ethanol exposure and withdrawal. METHODS: In the current study, extracellular levels of glutamate and glutamate transport in the nucleus accumbens were assayed 24 hr after repeated ethanol exposure (1 g/kg ip daily for 7 days) with use of in vivo no-net-flux microdialysis and in vitro [(3)H]glutamate uptake, respectively. RESULTS: Microdialysis revealed higher extracellular glutamate concentrations in the nucleus accumbens of rats that were given ethanol. The increase in basal extracellular glutamate levels was accounted for in part by a decrease in the in vivo probe recovery of glutamate. Moreover, an in vitro accumbens slice preparation measuring [(3)H]glutamate uptake revealed that Na(+)-dependent [(3)H]glutamate uptake was significantly reduced 24 hr after 7 days of repeated ethanol exposure. The ethanol-induced deficit in glutamate uptake was not associated with decreased total tissue levels of the transporters GLAST or GLT1. The in vivo and in vitro ethanol-induced changes in glutamate levels and uptake returned to control levels 14 days after discontinuing 7 days of repeated ethanol exposure. CONCLUSIONS: These results suggest that the previously reported increases in extracellular glutamate induced by ethanol exposure may be due in part to deficits in glutamate transport.
Roberto I Melendez; Megan P Hicks; Stephanie S Cagle; Peter W Kalivas
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Alcoholism, clinical and experimental research     Volume:  29     ISSN:  0145-6008     ISO Abbreviation:  Alcohol. Clin. Exp. Res.     Publication Date:  2005 Mar 
Date Detail:
Created Date:  2005-03-16     Completed Date:  2005-06-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7707242     Medline TA:  Alcohol Clin Exp Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  326-33     Citation Subset:  IM    
Department of Physiology and Neuroscience, Medical University of South Carolina, 173 Ashley Avenue, Charleston, SC 29425, USA.
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MeSH Terms
Actins / metabolism
Amino Acid Transport System X-AG / metabolism
Blotting, Western
Central Nervous System Depressants / pharmacology*
Ethanol / pharmacology*
Excitatory Amino Acid Transporter 2 / metabolism
Glutamic Acid / metabolism*
Nucleus Accumbens / drug effects,  metabolism*
Rats, Sprague-Dawley
Reg. No./Substance:
0/Actins; 0/Amino Acid Transport System X-AG; 0/Central Nervous System Depressants; 0/Excitatory Amino Acid Transporter 2; 56-86-0/Glutamic Acid; 64-17-5/Ethanol

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