Document Detail


Ethanol exposure affects cell movement during gastrulation and induces split axes in zebrafish embryos.
MedLine Citation:
PMID:  20394815     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To explore the toxic effects of ethanol on axis formation during embryogenesis, zebrafish embryos at different developmental stages were treated with 3% ethanol for 3h. The effects of ethanol exposure appeared to be stage-dependent. The dome stage embryo was most sensible to form posterior split axes upon ethanol exposure. Morphological and histological observations and whole-mount in situ hybridization results showed that ethanol exposure at this stage caused a general gastrulation delay, and induced double notochords, double neural tubes and two sets of somites in the posterior trunk. Mechanistically, no ectopic organizer was found by examining the expression patterns of dorsoventral markers including goosecoid, chordin and eve1 at the onset of gastrulation. However, radial intercalation, epiboly and convergence extension were inhibited by ethanol exposure as revealed by cell labeling, phenotypic observation and the expression patterns of axial or paraxial markers. Further investigation showed that the cell aggregation might be affected by ethanol exposure, as indicated by the much more scattered expression pattern of chordin, eve1 and wnt11 at the early gastrula stage, and the discontinuous gsc positive cells during migration. These results imply that ethanol might affect cell movement before and during gastrulation and as a consequence, induces a split axes phenotype.
Authors:
Ying Zhang; Ming Shao; Lifeng Wang; Zhongzhen Liu; Ming Gao; Chao Liu; Hongwei Zhang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-04-13
Journal Detail:
Title:  International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience     Volume:  28     ISSN:  1873-474X     ISO Abbreviation:  Int. J. Dev. Neurosci.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-05-17     Completed Date:  2010-08-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8401784     Medline TA:  Int J Dev Neurosci     Country:  England    
Other Details:
Languages:  eng     Pagination:  283-8     Citation Subset:  IM    
Copyright Information:
Copyright 2010 ISDN. Published by Elsevier Ltd. All rights reserved.
Affiliation:
Institute of Developmental Biology, College of Life Science, Key Lab of Experimental Teratology of Ministry of Education, Shandong University, 27 Shanda South Road, Jinan, Shandong 250100, China. zhangying9268@163.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Basic Helix-Loop-Helix Transcription Factors / genetics,  metabolism
Body Patterning / drug effects
Cell Movement / drug effects*
Embryo, Nonmammalian / drug effects,  metabolism
Ethanol / pharmacology*
Gastrulation / drug effects*
Gene Expression Regulation, Developmental / drug effects
In Situ Hybridization
Nerve Tissue Proteins / genetics,  metabolism
Phenotype
Species Specificity
T-Box Domain Proteins / genetics,  metabolism
Zebrafish / embryology*,  genetics,  metabolism
Zebrafish Proteins / genetics,  metabolism
Chemical
Reg. No./Substance:
0/Basic Helix-Loop-Helix Transcription Factors; 0/Nerve Tissue Proteins; 0/T-Box Domain Proteins; 0/Zebrafish Proteins; 0/neurog1 protein, zebrafish; 0/ntl protein, zebrafish; 64-17-5/Ethanol

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