Document Detail

Ethanol causes accelerated G1 arrest in differentiating HL-60 cells.
MedLine Citation:
PMID:  1702269     Owner:  NLM     Status:  MEDLINE    
The effects of clinically relevant ethanol concentrations on myeloid differentiation in the HL-60 cell promyelocytic leukemia line have been studied. The exposure of noninduced stem cells to 60 mM ethanol results in an increase in G1 cells, but there is no increase in superoxide production or expression of the Mo1 antigen. When HL-60 cells are induced to differentiate along the myeloid line with dimethylsulfoxide (DMSO) or retinoic acid (RA), there is a shift to smaller cell size, an increase in G1 cells and acquisition of the ability to produce superoxide as reported previously by several authors. When ethanol is present during differentiation, there are further increases in G1 cells, and increases in the percentage of cells which produce superoxide and express Mo1, and decreases in mean cell size and total growth during the incubation period. Regrowth experiments after periods of differentiation indicate that the increased G1 arrest seen in the presence of ethanol represents terminal commitment if inducer is present, but in the absence of inducer the increased G1 percentage is readily reversible. Examination of RNA content by flow cytometry reveals a decrease in both the peak and mean G1 RNA content during DMSO or RA induced differentiation. These decreases are accentuated by the presence of ethanol, resulting in a higher G1A/G1B ratio than in nonexposed cells. These findings indicate that ethanol enhances G1 growth arrest in HL-60 cells exposed to myeloid inducers. Partial differentiation occurs during this process, resulting in terminally arrested cells, some of which have undergone fewer postinduction cell divisions than normal and may not be fully competent.
R T Cook; J A Keiner; A Yen
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Alcoholism, clinical and experimental research     Volume:  14     ISSN:  0145-6008     ISO Abbreviation:  Alcohol. Clin. Exp. Res.     Publication Date:  1990 Oct 
Date Detail:
Created Date:  1991-02-01     Completed Date:  1991-02-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7707242     Medline TA:  Alcohol Clin Exp Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  695-703     Citation Subset:  IM    
Department of Pathology, Department of Veterans Affairs Medical Center, Iowa City, Iowa.
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MeSH Terms
Cell Differentiation / drug effects*
Cell Division / drug effects*
Cell Line
DNA Replication / drug effects
Dimethyl Sulfoxide / pharmacology
Ethanol / pharmacology*
G1 Phase / drug effects*
Leukemia, Promyelocytic, Acute
Monocytes / drug effects
RNA / drug effects
Superoxides / metabolism
Tretinoin / pharmacology
Tumor Cells, Cultured / drug effects*
Reg. No./Substance:
11062-77-4/Superoxides; 302-79-4/Tretinoin; 63231-63-0/RNA; 64-17-5/Ethanol; 67-68-5/Dimethyl Sulfoxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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