Document Detail

Ethanol administration and the relationship of malonyl-coenzyme A concentrations to the rate of fatty acid synthesis in rat liver.
MedLine Citation:
PMID:  4149946     Owner:  NLM     Status:  MEDLINE    
1. The effect of ethanol on liver fatty acid synthesis was studied in vivo in 24h-starved and ;meal-fed' rats (i.e. fed for 3h per day and not ad libitum). 2. In the fed animal (3)H(2)O was incorporated into fat at a rate of 0.46mumol of C(2) units/min per g wet wt. of liver. Administration of either ethanol (3.2g/kg) or equicaloric amounts of glucose had no effect on the rate of (3)H(2)O incorporation into lipid. 3. In the 24h-starved animal, administration of the same dose of ethanol produced an increase in the rate of (3)H(2)O incorporation from 0.06 to 0.12mumol of C(2) units/min per g fresh wt. after 3h whereas [malonyl-CoA] increased from 0.006 to 0.009mumol/g. Glucose given in amounts equicaloric to ethanol was significantly more lipogenic, increasing both the (3)H(2)O incorporation from 0.06 to 0.20mumol of C(2) units/min per g and the malonyl-CoA content from 0.006 to 0.013 mumol/g wet wt. at 3h. 4. The decrease in the redox state of free cytoplasm NAD or NADP couples or the changes in content of citrate, glucose 6-phosphate and pyruvate of liver after ethanol administration had no measurable effect on the rate of fatty acid synthesis in vivo. 5. Under the conditions of the experiments there was no significant difference, among any of the groups, in the activity of liver fatty acid synthetase measured in vitro. A double-reciprocal plot of the rate of (3)H(2)O incorporation and the total tissue malonyl-CoA concentrations showed a striking relationship. It has been concluded that the rate of fatty acid synthesis in vivo is determined principally by the V(max.) of fatty acid synthetase and the concentration of free malonyl-CoA. 6. It has also been concluded that under the conditions of the present study, the synthesis of fatty acids de novo is unlikely to be an important factor in the increased liver lipid content associated with ethanol administration.
R W Guynn; D Veloso; R L Harris; J W Lawson; R L Veech
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Biochemical journal     Volume:  136     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1973 Nov 
Date Detail:
Created Date:  1974-04-25     Completed Date:  1974-04-25     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  639-47     Citation Subset:  IM    
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MeSH Terms
Acetyl-CoA Carboxylase / metabolism
Citrates / metabolism
Coenzyme A / metabolism*
Ethanol / pharmacology*
Fatty Acid Synthetase Complex / metabolism
Fatty Acids / biosynthesis*
Glucose / pharmacology
Glucosephosphates / metabolism
Liver / enzymology,  metabolism*
Malonates / metabolism
Pyruvates / metabolism
Starvation / metabolism
Water / metabolism
Reg. No./Substance:
0/Citrates; 0/Fatty Acids; 0/Glucosephosphates; 0/Malonates; 0/Pyruvates; 10028-17-8/Tritium; 50-99-7/Glucose; 53-84-9/NAD; 64-17-5/Ethanol; 7732-18-5/Water; 85-61-0/Coenzyme A; EC 6.-/Fatty Acid Synthetase Complex; EC Carboxylase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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