| Ethanol inhibits gastric acid secretion in rats through increased AMP-kinase activity. | |
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MedLine Citation:
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PMID: 20110680 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effects of ethanol on gastric acid secretion remain controversial. The present study examines the effect of low-dose (2%) short term (15-20 min) ethanol exposure on gastric acid secretion via a potential interaction with AMP-activated protein kinase (AMPK). Real-time fluorescence digital imaging was used to provide functional evidence for the interaction of ethanol and AMPK in modulating secretagogue-induced acid secretion. Individual rat gastric glands were loaded with the pH-sensitive dye BCECF and the secretagogues carbachol (200 microM) or histamine (200 microM) were added to induce secretion. Rates of pH recovery were calculated as DeltapH(i)/Deltat. In one series of experiments, secretagogue-induced acid secretion was inhibited by 2% ethanol, or the AMPK activator AICAR monophosphate (AICAR) (20 mM). In a separate series, 2% ethanol was added in combination with compound C (20 microM), an AMPK inhibitor, to prevent activation of AMPK. 2% ethanol significantly suppressed stimulated acid secretion. In order to confirm modulation of AMPK activity by ethanol, the specific AMPK inhibitor compound C was used, which reversed the inhibitory effects of ethanol on stimulated acid secretion. This study demonstrates that low dose ethanol (2%) inhibits secretagogue-dependent acid secretion by activation of the AMPK pathway in rat gastric parietal cells. |
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Authors:
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Sascha Kopic; Stefanie Corradini; Shafik Sidani; Michael Murek; Artur Vardanyan; Michael F?ller; Markus Ritter; John P Geibel |
Publication Detail:
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Type: Journal Article Date: 2010-01-12 |
Journal Detail:
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Title: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology Volume: 25 ISSN: 1421-9778 ISO Abbreviation: Cell. Physiol. Biochem. Publication Date: 2010 |
Date Detail:
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Created Date: 2010-01-29 Completed Date: 2010-04-08 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9113221 Medline TA: Cell Physiol Biochem Country: Switzerland |
Other Details:
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Languages: eng Pagination: 195-202 Citation Subset: IM |
Copyright Information:
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Copyright 2010 S. Karger AG, Basel. |
Affiliation:
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Department of Surgery, Yale University, School of Medicine, New Haven, CT 06511, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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AMP-Activated Protein Kinases
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antagonists & inhibitors,
metabolism* Aminoimidazole Carboxamide / analogs & derivatives, pharmacology Animals Carbachol / pharmacology Ethanol / pharmacology* Fluoresceins / chemistry Fluorescent Dyes / chemistry Gastric Acid / secretion* Gastric Mucosa / metabolism, pathology Histamine / pharmacology Hydrogen-Ion Concentration Hypoglycemic Agents / pharmacology Rats Rats, Sprague-Dawley Ribonucleotides / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Fluoresceins; 0/Fluorescent Dyes; 0/Hypoglycemic Agents; 0/Ribonucleotides; 3031-94-5/AICA ribonucleotide; 360-97-4/Aminoimidazole Carboxamide; 51-45-6/Histamine; 51-83-2/Carbachol; 64-17-5/Ethanol; 85138-49-4/2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein; EC 2.7.11.1/AMP-Activated Protein Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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