Document Detail


Ethanol inhibits gastric acid secretion in rats through increased AMP-kinase activity.
MedLine Citation:
PMID:  20110680     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effects of ethanol on gastric acid secretion remain controversial. The present study examines the effect of low-dose (2%) short term (15-20 min) ethanol exposure on gastric acid secretion via a potential interaction with AMP-activated protein kinase (AMPK). Real-time fluorescence digital imaging was used to provide functional evidence for the interaction of ethanol and AMPK in modulating secretagogue-induced acid secretion. Individual rat gastric glands were loaded with the pH-sensitive dye BCECF and the secretagogues carbachol (200 microM) or histamine (200 microM) were added to induce secretion. Rates of pH recovery were calculated as DeltapH(i)/Deltat. In one series of experiments, secretagogue-induced acid secretion was inhibited by 2% ethanol, or the AMPK activator AICAR monophosphate (AICAR) (20 mM). In a separate series, 2% ethanol was added in combination with compound C (20 microM), an AMPK inhibitor, to prevent activation of AMPK. 2% ethanol significantly suppressed stimulated acid secretion. In order to confirm modulation of AMPK activity by ethanol, the specific AMPK inhibitor compound C was used, which reversed the inhibitory effects of ethanol on stimulated acid secretion. This study demonstrates that low dose ethanol (2%) inhibits secretagogue-dependent acid secretion by activation of the AMPK pathway in rat gastric parietal cells.
Authors:
Sascha Kopic; Stefanie Corradini; Shafik Sidani; Michael Murek; Artur Vardanyan; Michael F?ller; Markus Ritter; John P Geibel
Publication Detail:
Type:  Journal Article     Date:  2010-01-12
Journal Detail:
Title:  Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology     Volume:  25     ISSN:  1421-9778     ISO Abbreviation:  Cell. Physiol. Biochem.     Publication Date:  2010  
Date Detail:
Created Date:  2010-01-29     Completed Date:  2010-04-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9113221     Medline TA:  Cell Physiol Biochem     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  195-202     Citation Subset:  IM    
Copyright Information:
Copyright 2010 S. Karger AG, Basel.
Affiliation:
Department of Surgery, Yale University, School of Medicine, New Haven, CT 06511, USA.
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MeSH Terms
Descriptor/Qualifier:
AMP-Activated Protein Kinases / antagonists & inhibitors,  metabolism*
Aminoimidazole Carboxamide / analogs & derivatives,  pharmacology
Animals
Carbachol / pharmacology
Ethanol / pharmacology*
Fluoresceins / chemistry
Fluorescent Dyes / chemistry
Gastric Acid / secretion*
Gastric Mucosa / metabolism,  pathology
Histamine / pharmacology
Hydrogen-Ion Concentration
Hypoglycemic Agents / pharmacology
Rats
Rats, Sprague-Dawley
Ribonucleotides / pharmacology
Chemical
Reg. No./Substance:
0/Fluoresceins; 0/Fluorescent Dyes; 0/Hypoglycemic Agents; 0/Ribonucleotides; 3031-94-5/AICA ribonucleotide; 360-97-4/Aminoimidazole Carboxamide; 51-45-6/Histamine; 51-83-2/Carbachol; 64-17-5/Ethanol; 85138-49-4/2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein; EC 2.7.11.1/AMP-Activated Protein Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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