| Ethanol-induced alterations in fatty acid-related lipids in serum and tissues in mice. | |
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MedLine Citation:
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PMID: 21058963 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Chronic alcohol consumption is a major factor for several human diseases, and alcoholism is associated with a host of societal problems. One of the major alcohol-induced metabolic changes is the increased NADH levels, which reduces glucose synthesis and increases fatty acid (FA) synthesis. Probably more important is the induction of FA synthesizing enzymes under the control of sterol regulatory element binding proteins (SREBP), plus increased malonyl-CoA, which blocks FA entry to the mitochondria for oxidation. The changes in FA-related lipids, particularly lysophospholipids and ceramides (Cers), in different tissues in ethanol-fed mice have not been reported. METHODS: We systematically determined the levels of FA-related lipids, including FAs, phosphatidylcholines, phosphatidylethanolamines, lysophosphatidic acid, lysophosphatidylcholine, lysophosphatidylethanolamine, lysophosphatidylinositol, sphingomyelins, and ceramides (Cers), in the serum and different tissues by high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (HPLC-ESI-MS/MS). The study was performed in C57BL/6J mice fed with Lieber-DeCarli diet, in which ethanol was added to account for 27.5% of total calories. The serum and tissues were collected from these mice at the time of killing, and the results were compared to pair-fed controls. RESULTS: The important observation was that ethanol-induced tissue-specific changes, which were related to different FA chains. Several 22:6 FA, 18:0 FA, 18:0 to 18:3 FA-containing lipids were significantly increased in the serum, liver, and skeletal muscle, respectively. In the kidney, all 22:6 FA-containing lipids detected were increased. In addition, alterations in other lipids in tissues, except adipose tissue, were also observed. CONCLUSIONS: We found tissue-specific alterations in the levels of FA-related lipids after ethanol administration. The implications of these findings pertinent to human physiology/pathology warrant further investigation. More studies are needed to explore the mechanisms on the different effects of ethanol on certain lipids in different tissues. |
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Authors:
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Zhenwen Zhao; Menggang Yu; David Crabb; Yan Xu; Suthat Liangpunsakul |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-11-08 |
Journal Detail:
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Title: Alcoholism, clinical and experimental research Volume: 35 ISSN: 1530-0277 ISO Abbreviation: Alcohol. Clin. Exp. Res. Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-01-25 Completed Date: 2011-07-29 Revised Date: 2012-02-02 |
Medline Journal Info:
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Nlm Unique ID: 7707242 Medline TA: Alcohol Clin Exp Res Country: England |
Other Details:
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Languages: eng Pagination: 229-34 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 by the Research Society on Alcoholism. |
Affiliation:
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Department of Obstetrics and Gynecology, Indiana University School of Medicine, Indianapolis, USA. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Alcoholism
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metabolism* Animals Ethanol / administration & dosage* Fatty Acids / blood, metabolism* Humans Kidney / drug effects, metabolism* Lipid Metabolism* Lipids / blood Lipogenesis Liver / drug effects, metabolism* Male Mice Mice, Inbred C57BL Muscle, Skeletal / drug effects, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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K08 AA016570-02/AA/NIAAA NIH HHS; K08AA016570/AA/NIAAA NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Lipids; 64-17-5/Ethanol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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