Document Detail


Eternal youth, the fate of developing Arabidopsis leaves upon Rhodococcus fascians infection.
MedLine Citation:
PMID:  19118126     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The phytopathogenic actinomycete Rhodococcus fascians induces neoplastic shooty outgrowths on infected hosts. Upon R. fascians infection of Arabidopsis (Arabidopsis thaliana), leaves are formed with small narrow lamina and serrated margins. These symptomatic leaves exhibit reduced tissue differentiation, display more but smaller cells that do not endoreduplicate, and accumulate in the G1 phase of the cell cycle. Together, these features imply that leaf growth occurs primarily through mitotic cell division and not via cell expansion. Molecular analysis revealed that cell cycle gene expression is activated continuously throughout symptomatic leaf development, ensuring persistent mitotic cycling and inhibition of cell cycle exit. The transition at the two major cell cycle checkpoints is stimulated as a direct consequence of the R. fascians signals. The extremely reduced phenotypical response of a cyclind3;1-3 triple knockout mutant indicates that the D-type cyclin/retinoblastoma/E2F transcription factor pathway, as a major mediator of cell growth and cell cycle progression, plays a key role in symptom development and is instrumental for the sustained G1-to-S and G2-to-M transitions during symptomatic leaf growth.
Authors:
Stephen Depuydt; Lieven De Veylder; Marcelle Holsters; Danny Vereecke
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-12-31
Journal Detail:
Title:  Plant physiology     Volume:  149     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-03-04     Completed Date:  2009-05-14     Revised Date:  2010-09-23    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1387-98     Citation Subset:  IM    
Affiliation:
Department of Plant Systems Biology, Flanders Institute for Biotechnology, Belgium.
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MeSH Terms
Descriptor/Qualifier:
Arabidopsis / cytology,  genetics,  growth & development*,  microbiology*
Arabidopsis Proteins / metabolism
Cell Differentiation
Cell Proliferation
Cyclins / metabolism
DNA, Plant / metabolism
G1 Phase
Genes, Plant
Mitosis / genetics
Models, Biological
Phenotype
Plant Epidermis / cytology,  metabolism,  microbiology
Plant Leaves / cytology,  genetics,  growth & development*,  microbiology*
Ploidies
Rhodococcus / cytology,  physiology*
S Phase
Transcription, Genetic
Chemical
Reg. No./Substance:
0/Arabidopsis Proteins; 0/CycD3 protein, Arabidopsis; 0/Cyclins; 0/DNA, Plant
Comments/Corrections

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