Document Detail

Estrogens in maternal plasma following intraamniotic injection of (3H)-dehydroepiandrosterone-sulfate in midpregnancy.
MedLine Citation:
PMID:  137965     Owner:  NLM     Status:  MEDLINE    
In 4 patients with normal pregnancies between the 18th and 20th week of gestation (3H7alpha)-dehydroepiandrosterone-sulfate ([H]-DHEA-S) was injected intraamniotically. Maternal venous blood was drawn before and at regular intervals for 240 minutes after DHEA-injection. Thereafter, legal abortion was performed by intraamniotic instillation of prostaglandine. The conjugated steroids were hydrolyzed enzymatically and the total steroids were isolated and identified. The following labelled metabolites were determined quantitatively: Estriol (e3, estradiol-17beta (E2-17beta), estrone(E1), 16alpha-hydroxy-estrone, (16alpha-OH-DHEA), ALPHA4-androstenedione (AD) and testosterone (T). The maximal increase of all estrogen fractions in matermal plasma occurred 120-180min after intraamniotic injection of the precursor. The most prominent rise of the C18-steroids could be shown for estriol. 60-70% of all metabolites were C16-hydroxylated.
Tritiated-7alpha-dehydroepiandrosterone sulfate (DHEA-S) was injected intraamniotically in 4 women with normal pregnancies between the 18th-20th week of gestation, and its metabolities were determined. Maternal venous blood samples were obtained before and at regular intervals for 4 hours after injection of DHEA-S. Abortion was then induced by intraamniotic instillation of prostaglandins. The following labeled metabolities were identified: estriol, estradiol-17beta, estrone, 16alpha-hydroxy-estrone, dehydroepiandrosterone, delta4-androstenedione and testosterone. All estrogen fractions showed a maximal increase in maternal plasma between 120-180 minutes after injection of DHEA-S. Of the c(18)-steroids, estriol showed the greatest increase. Approximately 60-70% of all the metabolites were c(16)-hydroxylated. The results provide valuable information regarding the quantity of fetoplacental steroid metabolism in midpregnancy.
W D Lehmann; J R Strecker
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of perinatal medicine     Volume:  4     ISSN:  0300-5577     ISO Abbreviation:  J Perinat Med     Publication Date:  1976  
Date Detail:
Created Date:  1977-03-21     Completed Date:  1977-03-21     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0361031     Medline TA:  J Perinat Med     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  255-60     Citation Subset:  IM; J    
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MeSH Terms
Androgens / blood
Dehydroepiandrosterone / administration & dosage,  diagnostic use*,  metabolism
Estrogens / blood*
Placenta / metabolism
Pregnancy Trimester, Second
Reg. No./Substance:
0/Androgens; 0/Estrogens; 53-43-0/Dehydroepiandrosterone

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