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Estrogenic activity of natural and synthetic estrogens in human breast cancer cells in culture.
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MedLine Citation:
PMID:  9168008     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We investigated the estrogenic activity of various environmental pollutants (xenobiotics), in particular the xenoestrogen o,p-DDT, and compared their effects with those of endogenous estrogens, phytoestrogens, and mycoestrogens on estrogen receptor binding capacity, induction of estrogen end products, and activation of cell proliferation in estrogen-sensitive human breast cancer cells in monolayer culture. We also quantified the levels of phytoestrogens in extracts of some common foods, herbs, and spices and in human saliva following consumption of a high phytoestrogen food source (soy milk) to compare phytoestrogen abundance and bioavailability relative to the reported xenoestrogen burden in humans. Results show that natural endogenous estrogens, phytoestrogens, mycoestrogens, and xenoestrogens bind estrogen receptor (ER) in intact cells, but demonstrate marked differences in their ability to induce end products of estrogen action and to regulate cell proliferation. All of the different classes of estrogens stimulated cell proliferation at concentrations that half-saturated ER, but only some classes were able to induce estrogen-regulated end products. Genistein, a common phytoestrogen found in soy foods, differed from the xenoestrogen DDT in its effects on cell proliferation and ability to induce estrogen-regulated end products. Moreover, we found that many of the foods, herbs, and spices commonly consumed by humans contain significant amounts of phytoestrogens, and consumption of soy milk, a phytoestrogen-rich food, markedly increases the levels of phytoestrogens in saliva. In conclusion, our in vitro results predict that a diet high in phytoestrogens would significantly reduce the binding of weak xenoestrogens to ER in target tissues in vivo.
Authors:
D T Zava; M Blen; G Duwe
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Environmental health perspectives     Volume:  105 Suppl 3     ISSN:  0091-6765     ISO Abbreviation:  Environ. Health Perspect.     Publication Date:  1997 Apr 
Date Detail:
Created Date:  1997-08-11     Completed Date:  1997-08-11     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0330411     Medline TA:  Environ Health Perspect     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  637-45     Citation Subset:  IM    
Affiliation:
Aeron Biotechnology, San Leandro, California 94577, USA. aeron@aeron.com
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MeSH Terms
Descriptor/Qualifier:
Breast Neoplasms / etiology*,  metabolism,  pathology
Cell Division / drug effects
DDT / metabolism,  toxicity
Diet
Environmental Health
Environmental Pollutants / metabolism,  toxicity
Estradiol Congeners / metabolism,  toxicity*
Estrogens / metabolism,  toxicity*
Estrogens, Non-Steroidal / metabolism,  pharmacology,  toxicity*
Female
Food Analysis
Humans
Isoflavones*
Neoplasms, Hormone-Dependent / etiology,  metabolism,  pathology
Phytoestrogens
Plant Preparations
Receptors, Estrogen / drug effects,  metabolism
Saliva / metabolism
Tumor Cells, Cultured
Grant Support
ID/Acronym/Agency:
2R44 HD27738-02/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Environmental Pollutants; 0/Estradiol Congeners; 0/Estrogens; 0/Estrogens, Non-Steroidal; 0/Isoflavones; 0/Phytoestrogens; 0/Plant Preparations; 0/Receptors, Estrogen; 50-29-3/DDT; 789-02-6/o,p'-DDT
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): Environ Health Perspect
ISSN: 0091-6765
Article Information
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Print publication date: Month: 4 Year: 1997
Volume: 105 Issue: Suppl 3
First Page: 637 Last Page: 645
ID: 1469890
PubMed Id: 9168008

Estrogenic activity of natural and synthetic estrogens in human breast cancer cells in culture.
D T Zava
M Blen
G Duwe
Aeron Biotechnology, San Leandro, California 94577, USA. aeron@aeron.com



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  • Research Article


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