Document Detail

Estrogen up-regulates MMP2/9 expression in endometrial epithelial cell via VEGF-ERK1/2 pathway.
MedLine Citation:
PMID:  23870474     Owner:  NLM     Status:  In-Data-Review    
OBJECTIVE: To study the effect of estrogen on anovulatory dysfunctional uterine bleeding (ADUB).
METHODS: Primary endometrial epithelial cells of Hainan Lizu female was cultured and hydrolytic activity of gelatinase was determined by gelatin zymography analysis. Cellular mRNA and protein synthesis was blocked respectively to determine whether the increased expression of MMP-2/9 was induced by estrogen. The expression of VEGF was blocked by siRNA. After treatment with various factors, MMP-9, VEGF, total Erk and phosphorylated Erk expression in primary uterine epithelial cells was detected by Western blotting analysis. Cell MMP-2/9mRNA levels was measured by real-time RT-PCR.
RESULTS: The activity and expression of MMP2/9 was increased in the endometrium of patients with ADUB. Estrogen could up-regulate the expression of VEGF and activate Erk 1/2-Elk1 signal path. After interference by siRNA, ERK1/2 pathway was blocked in cells, and the expression of MMP-2/9 was down-regulated. ERK1/2 specific blocker U0126 blocked ERK phosphorylation, and it could down-regulate the expression of MMP-2/9.
CONCLUSIONS: The results showed that the estrogen can increase the expression of VEGF, and thus activate ERK1/2 pathway to induce MMP-2/9 expression.
Bao Shan; Wang Li; Shu-Ying Yang; Zhuo-Ri Li
Related Documents :
24449344 - Importance of sox8 for in vitro chondrogenic differentiation of human mesenchymal strom...
23536684 - The transcriptome of the baculovirus autographa californica multiple nucleopolyhedrovir...
24013584 - Mir-106a-5p inhibits the proliferation and migration of astrocytoma cells and promotes ...
24793974 - Hyperglycemia impairs cytotrophoblast function via stress signaling.
18490444 - Mkp-1 mrna stabilization and translational control by rna-binding proteins hur and nf90.
18579444 - Expression of mt2 and smt-b upon cadmium exposure and cold shock in zebrafish (danio re...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Asian Pacific journal of tropical medicine     Volume:  6     ISSN:  1995-7645     ISO Abbreviation:  Asian Pac J Trop Med     Publication Date:  2013 Oct 
Date Detail:
Created Date:  2013-07-22     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101533720     Medline TA:  Asian Pac J Trop Med     Country:  Singapore    
Other Details:
Languages:  eng     Pagination:  826-30     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 Hainan Medical College. Published by Elsevier B.V. All rights reserved.
Department of Gynaecology and Obstetrics of Hainan People's Hospital, Hai Kou 570311, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Brucellosis and tuberculosis: Clinical overlap and pitfalls.
Next Document:  Expression and significance of p53 and mdm2 in patients with leukoplakia cancer.