Document Detail

Estrogen stimulates expression of p21Waf1/Cip1 in mouse uterine luminal epithelium.
MedLine Citation:
PMID:  12108525     Owner:  NLM     Status:  MEDLINE    
p21Waf1/Cip1 was originally identified as an inhibitor of the cell cycle. Recent evidence suggests that it can act as a positive regulator of the cell cycle under the influence of some growth stimulators. We investigated the effects of ovarian steroids on the expression of p21, DNA synthesis, and mitosis in the uterus. Capsules containing 17beta-estradiol (E2) were subcutaneously implanted in ovariectomized mice that were sacrificed on different days. Their uteri were collected for p21 immunohistochemical staining. To study mitosis and DNA synthesis, colchicine and bromodeoxyuridine (BrdU) were injected into mice 3 or 5 h before sacrifice. The results showed that p21 expression, BrdU incorporation, and the mitotic index in uterine luminal epithelium increased 1 to 2 d after E2 stimulation and then declined to basal levels between d 3 and 6. Furthermore, cotreatment with progesterone (P4) and E2 suppressed both p21 expression and the DNA synthesis stimulated by E2 alone in uterine epithelial cells. Our results show that estrogen stimulates p21 expression and cell proliferation in uterine luminal epithelium and that cotreatment with P4 prevents both effects, suggesting that p21 may act as a positive cell-cycle regulator.
Ming-Derg Lai; Meei Jyh Jiang; Lih-Yuh C Wing
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Endocrine     Volume:  17     ISSN:  1355-008X     ISO Abbreviation:  Endocrine     Publication Date:  2002 Apr 
Date Detail:
Created Date:  2002-07-10     Completed Date:  2002-12-24     Revised Date:  2010-06-24    
Medline Journal Info:
Nlm Unique ID:  9434444     Medline TA:  Endocrine     Country:  United States    
Other Details:
Languages:  eng     Pagination:  233-9     Citation Subset:  IM    
Department of Physiology, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
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MeSH Terms
Antimetabolites / diagnostic use
Bromodeoxyuridine / diagnostic use
Coloring Agents
Cyclin A / biosynthesis,  genetics
Cyclin D1 / biosynthesis,  genetics
Cyclin-Dependent Kinase Inhibitor p21
Cyclins / biosynthesis*
DNA / biosynthesis
Epithelium / drug effects,  metabolism
Estrogens / pharmacology*
Mice, Inbred C57BL
Mitosis / drug effects
Progesterone / pharmacology
Uterus / drug effects,  metabolism*
Reg. No./Substance:
0/Antimetabolites; 0/Cdkn1a protein, mouse; 0/Coloring Agents; 0/Cyclin A; 0/Cyclin-Dependent Kinase Inhibitor p21; 0/Cyclins; 0/Estrogens; 136601-57-5/Cyclin D1; 57-83-0/Progesterone; 59-14-3/Bromodeoxyuridine; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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