Document Detail

Estrogen-responsive RING finger mRNA induction in gastrointestinal carcinoma cells following bile acid treatment.
MedLine Citation:
PMID:  9855000     Owner:  NLM     Status:  MEDLINE    
The underlying molecular mechanisms of the tumor-promoting activity of bile acids such as chenodeoxycholic acid (CDCA) and deoxycholic acid (DCA) and the protective effect of ursodeoxycholic acid (UDCA) remain largely unclear. Using RNA arbitrarily primed PCR (RAP-PCR) for differential display, we identified, cloned and sequenced differentially expressed transcripts after treating gastric carcinoma cells (St 23132) with the bile acids CDCA, DCA and UDCA. One of these transcripts was identified to be an estrogen-responsive RING finger protein (efp) mRNA. The differential expression of efp in gastric cancer cells was confirmed by low stringency RT-PCR. efp mRNA levels were induced 3-fold in gastric carcinoma cells after CDCA and DCA treatment, whereas no change in expression was detected after UDCA treatment. Finally, treatment of the colon carcinoma cell line HT 29 with DCA resulted in a 2- to 5-fold induction of efp mRNA levels whereas UDCA did not induce efp. As expected, efp expression was also increased after 24 h of estrogen treatment. In summary, a synergy or a common pathway of tumor enhancement of bile acids and estrogen via efp in gastrointestinal carcinogenesis can be envisioned.
B Jung; T Vogt; F Mathieu-Daudé; J Welsh; M McClelland; T Trenkle; C Weitzel; F Kullmann
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Carcinogenesis     Volume:  19     ISSN:  0143-3334     ISO Abbreviation:  Carcinogenesis     Publication Date:  1998 Nov 
Date Detail:
Created Date:  1998-12-23     Completed Date:  1998-12-23     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8008055     Medline TA:  Carcinogenesis     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1901-6     Citation Subset:  IM    
Sidney Kimmel Cancer Center, San Diego, CA 92121, USA.
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MeSH Terms
Bile Acids and Salts / pharmacology*
Chenodeoxycholic Acid / pharmacology
DNA-Binding Proteins / genetics*
Deoxycholic Acid / pharmacology
Estrogens / pharmacology*
Gastrointestinal Neoplasms / etiology,  metabolism*
RNA, Messenger / analysis*
Transcription Factors / genetics*
Tumor Cells, Cultured
Ubiquitin-Protein Ligases
Ursodeoxycholic Acid / pharmacology
Zinc Fingers*
Reg. No./Substance:
0/Bile Acids and Salts; 0/DNA-Binding Proteins; 0/Estrogens; 0/RNA, Messenger; 0/Transcription Factors; 128-13-2/Ursodeoxycholic Acid; 474-25-9/Chenodeoxycholic Acid; 83-44-3/Deoxycholic Acid; EC protein, human; EC Ligases

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