Document Detail


Estrogen regulation of thecal cell steroidogenesis and differentiation: thecal cell-granulosa cell interactions.
MedLine Citation:
PMID:  2249634     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Estrogen regulation of thecal cell steroidogenesis and differentiation was investigated with cells from ovarian antral follicles. Bovine theca interna cells were isolated and cultured in serum-free conditions to evaluate the effects of estradiol on thecal cell production of androstenedione, testosterone, and progesterone. Estradiol increased thecal cell androgen production throughout a 6-day culture period; however, the basal and stimulated levels of androgen production diminished after day 3 of culture. Androstenedione accumulation was approximately 10-fold greater than that of testosterone. In contrast to the stimulatory effects that estradiol had on androgen production, estradiol suppressed progesterone production throughout the 6-day culture period. Comparison of the effects of estradiol and hCG on thecal cells from small (less than 5 mm), medium (5-10 mm), and large (greater than 10 mm) antral follicles demonstrated that estradiol stimulated androgen production to a greater extent than hCG with cells from all of these stages of follicle development. Estradiol stimulation of androstenedione was greater in theca from small follicles than in theca from medium or large follicles. In contrast, suppressive effects of estradiol on progesterone were most apparent on thecal cells from medium and large follicles and less apparent on theca from small follicles. Estradiol stimulated androstenedione production in a dose-dependent fashion, with a minimum effective concentration of 10(-9) M and a maximum effective concentration of 10(-7)-10(-6) M. Concentrations greater than 10(-6) M estradiol resulted in a decline in the stimulatory response and may be important in the preovulatory follicle to suppress thecal cell androgen production and initiate the process of luteinization. Progesterone production was slightly stimulated by 10(-9) M estradiol, whereas higher concentrations (10(-7)-5 x 10(-6) M) resulted in a dose-dependent suppression of progesterone production. Interestingly, combined treatment of thecal cells with estradiol and hCG resulted in a greater than additive stimulation of androstenedione production, and estradiol decreased the ability of hCG to stimulate progesterone production. Observations demonstrate that estradiol can dramatically alter thecal cell production of steroids and support a hypothesis that steroid-mediated interactions between granulosa and thecal cells play an important role in regulating cellular function within follicles. The data provide evidence that a local feedback loop may exist in ovarian follicles, where androgens produced by thecal cells are used as a substrate for granulosa cell aromatization into estrogens, which, in turn, may feed back to stimulate thecal cell production of androgens.(ABSTRACT TRUNCATED AT 400 WORDS)
Authors:
A J Roberts; M K Skinner
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Endocrinology     Volume:  127     ISSN:  0013-7227     ISO Abbreviation:  Endocrinology     Publication Date:  1990 Dec 
Date Detail:
Created Date:  1991-01-17     Completed Date:  1991-01-17     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2918-29     Citation Subset:  AIM; IM    
Affiliation:
Department of Pharmacology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-6600.
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MeSH Terms
Descriptor/Qualifier:
Androstenedione / biosynthesis*
Animals
Cattle
Cell Communication
Cells, Cultured
Chorionic Gonadotropin / pharmacology
DNA Replication / drug effects
Estradiol / pharmacology
Female
Granulosa Cells / physiology*
Kinetics
Progesterone / biosynthesis*
Theca Cells / cytology,  drug effects,  physiology*
Grant Support
ID/Acronym/Agency:
HD-20922/HD/NICHD NIH HHS; T32-HD-07043/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Chorionic Gonadotropin; 50-28-2/Estradiol; 57-83-0/Progesterone; 63-05-8/Androstenedione

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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