| Estrogen regulation of neurotrophin expression in sympathetic neurons and vascular targets. | |
| | |
MedLine Citation:
|
PMID: 17289002 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We hypothesize that estrogen exerts a modulatory effect on sympathetic neurons to reduce neural cardiovascular tone and that these effects are modulated by nerve growth factor (NGF), a neurotrophin that regulates sympathetic neuron survival and maintenance. We examined the effects of estrogen on NGF and tyrosine hydroxylase (TH) protein content in specific vascular targets. Ovariectomized, adult Sprague-Dawley rats were implanted with placebo or 17beta-estradiol (release rate, 0.05 mg/day). Fourteen days later, NGF levels in the superior cervical ganglia (SCG) and its targets, the heart, external carotid artery, and the extracerebral blood vessels, as well as estrogen receptor alpha (ERalpha) content levels in the heart, were determined using semi-quantitative Western blot analysis. TH levels in the SCG and extracerebral blood vessels were determined by Western blotting and immunocytochemistry, respectively. Circulating levels of 17beta-estradiol and prolactin (PRL) were quantified by RIA. Estrogen replacement significantly decreased NGF protein in the SCG and its targets, the external carotid artery, heart and extracerebral blood vessels. TH protein associated with the extracerebral blood vessels was also significantly decreased, but ERalpha levels were significantly increased in the heart following estrogen replacement. These results indicate that estrogen reduces NGF protein content in sympathetic vascular targets, which may lead to decreased sympathetic innervations to these targets, and therefore reduced sympathetic regulation. In addition, the estrogen-induced increase in ERalpha levels in the heart, a target tissue of the SCG, suggests that estrogen may sensitize the heart to further estrogen modulation, and possibly increase vasodilation of the coronary vasculature. |
| | |
Authors:
|
Gurjinder Kaur; James Janik; Lori G Isaacson; Phyllis Callahan |
Publication Detail:
|
Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't Date: 2007-01-08 |
Journal Detail:
|
Title: Brain research Volume: 1139 ISSN: 0006-8993 ISO Abbreviation: Brain Res. Publication Date: 2007 Mar |
Date Detail:
|
Created Date: 2007-03-05 Completed Date: 2007-05-08 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0045503 Medline TA: Brain Res Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 6-14 Citation Subset: IM |
Affiliation:
|
Center for Neuroscience and Behavior, Department of Zoology, Miami University, Oxford, OH 45056, USA. gkaur@nki.rfmh.org |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adrenergic Fibers
/
metabolism Animals Brain / blood supply Carotid Artery, External / innervation, metabolism Cerebral Arteries / innervation, metabolism Down-Regulation Estradiol / physiology* Estrogen Receptor alpha / metabolism Estrogen Replacement Therapy Female Heart / innervation Immunohistochemistry Myocardium / metabolism Nerve Growth Factor / metabolism* Neurons / metabolism* Ovariectomy Prolactin / blood Protein Isoforms Rats Rats, Sprague-Dawley Superior Cervical Ganglion / cytology, metabolism* Tyrosine 3-Monooxygenase / metabolism* Vasodilation / physiology |
| Chemical | |
Reg. No./Substance:
|
0/Estrogen Receptor alpha; 0/Protein Isoforms; 50-28-2/Estradiol; 9002-62-4/Prolactin; 9061-61-4/Nerve Growth Factor; EC 1.14.16.2/Tyrosine 3-Monooxygenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Selective septohippocampal - but not forebrain amygdalar - cholinergic dysfunction in diencephalic a...
Next Document: Injection of mouse and human neural stem cells into neonatal Niemann-Pick A model mice.