Document Detail

Estrogen receptors, progestin receptors and DNA synthesis in the macaque endometrium during the luteal-follicular transition.
MedLine Citation:
PMID:  2278848     Owner:  NLM     Status:  MEDLINE    
We have suggested that in the nonhuman primate endometrium, stromal cells might play a role in mediating the effects of estrogen on the epithelium, especially during the luteal-follicular transition (LFT) when target cells normally escape from the inhibitory influence of progesterone (P). We now report that like estrogen receptors (ER), endometrial progestin receptors (PR) are detectable only in stromal cells until the fifth day of the LFT. With a technique that combined immunocytochemistry and autoradiography on the same sections, we characterized the cellular distribution of ER or PR coincidentally with the localization of [3H]thymidine taken up in vitro by endometria from monkeys undergoing an LFT. DNA synthesis in the glands of the upper endometrium was E2-dependent, but the distribution of [3H]thymidine was not positively correlated with the presence of ER or PR. Readministration of P to animals on days 3 or 4 of the LFT significantly reduced the [3H]thymidine labeling index of the glandular epithelium and caused stromal ER to decline, but P did not block the eventual appearance of ER in epithelial cells on day 5 of the LFT. Thus, E2 stimulated DNA synthesis in epithelial cells that lacked ER, and P suppressed DNA synthesis in these cells even though PR was only detected in the stroma when P treatment began. These data are consistent with a role for endometrial stromal cells in mediating the effects of E2 and P on the epithelium during the LFT.
M C McClellan; S Rankin; N B West; R M Brenner
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of steroid biochemistry and molecular biology     Volume:  37     ISSN:  0960-0760     ISO Abbreviation:  J. Steroid Biochem. Mol. Biol.     Publication Date:  1990 Dec 
Date Detail:
Created Date:  1991-03-08     Completed Date:  1991-03-08     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9015483     Medline TA:  J Steroid Biochem Mol Biol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  631-41     Citation Subset:  IM    
Reed College, Portland, OR 97202.
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MeSH Terms
Corpus Luteum / physiology
DNA / biosynthesis*
Endometrium / drug effects,  metabolism*,  ultrastructure
Epithelium / drug effects,  metabolism,  ultrastructure
Estrogens / pharmacology
Macaca fascicularis
Ovarian Follicle / physiology
Progesterone / pharmacology
Receptors, Estrogen / drug effects,  metabolism*
Receptors, Progesterone / drug effects,  metabolism*
Thymidine / metabolism
Grant Support
Reg. No./Substance:
0/Estrogens; 0/Receptors, Estrogen; 0/Receptors, Progesterone; 10028-17-8/Tritium; 50-89-5/Thymidine; 57-83-0/Progesterone; 9007-49-2/DNA

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