| Estrogen receptors alpha and beta in the rodent mammary gland. | |
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MedLine Citation:
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PMID: 10618419 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An obligatory role for estrogen in growth, development, and functions of the mammary gland is well established, but the roles of the two estrogen receptors remain unclear. With the use of specific antibodies, it was found that both estrogen receptors, ERalpha and ERbeta, are expressed in the rat mammary gland but the presence and cellular distribution of the two receptors are distinct. In prepubertal rats, ERalpha was detected in 40% of the epithelial cell nuclei. This decreased to 30% at puberty and continued to decrease throughout pregnancy to a low of 5% at day 14. During lactation there was a large induction of ERalpha with up to 70% of the nuclei positive at day 21. Approximately 60-70% of epithelial cells expressed ERbeta at all stages of breast development. Cells coexpressing ERalpha and ERbeta were rare during pregnancy, a proliferative phase, but they represented up to 60% of the epithelial cells during lactation, a postproliferative phase. Western blot analysis and sucrose gradient centrifugation confirmed this pattern of expression. During pregnancy, the proliferating cell nuclear antigen was not expressed in ERalpha-positive cells but was observed in 3-7% of ERbeta-containing cells. Because more than 90% of ERbeta-bearing cells do not proliferate, and 55-70% of the dividing cells have neither ERalpha nor ERbeta, it is clear that the presence of these receptors in epithelial cells is not a prerequisite for estrogen-mediated proliferation. |
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Authors:
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S Saji; E V Jensen; S Nilsson; T Rylander; M Warner; J A Gustafsson |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 97 ISSN: 0027-8424 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 2000 Jan |
Date Detail:
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Created Date: 2000-02-10 Completed Date: 2000-02-10 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 337-42 Citation Subset: IM |
Affiliation:
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Department of Medical Nutrition, Karolinska Institute, NOVUM, S-141 86 Huddinge, Sweden. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies / immunology Blotting, Western Cell Differentiation Cell Division Centrifugation, Density Gradient Estrogen Receptor alpha Estrogen Receptor beta Female Immunohistochemistry Lactation Male Mammary Glands, Animal / growth & development, metabolism* Pregnancy Prostate / metabolism Rats Rats, Sprague-Dawley Receptors, Estrogen / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Antibodies; 0/Estrogen Receptor alpha; 0/Estrogen Receptor beta; 0/Receptors, Estrogen |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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