| Estrogen receptor variants in epithelial compartment of normal human breast. | |
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MedLine Citation:
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PMID: 10963044 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Estrogen receptor (ER) splice variants have previously been identified in normal human breast. Normal breast, however, comprises many cell types including fat cells, fibroblasts, endothelial cells, and a variety of inflammatory cells besides breast epithelial cells. The objective of our study was to demonstrate the existence of several variants in the epithelial compartment of the normal human breast. To this end, highly enriched breast epithelial cells from reduction mammoplasty specimens were isolated using Percoll gradient centrifugation. We analyzed the presence of both wild-type (WT) and variant receptors in human breast epithelial cells using the seminested polymerase chain reaction and direct automated sequencing of the amplified products. We demonstrated that a number of spliced variants of the ER coexist with the WT receptor. Variants lacking exons 2, 4, 5, and 7 are detected in the breast epithelial compartment of the normal human breast. |
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Authors:
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J Yang; A Liu; R Guzman; S Nandi |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Endocrine Volume: 12 ISSN: 1355-008X ISO Abbreviation: Endocrine Publication Date: 2000 Jun |
Date Detail:
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Created Date: 2000-11-29 Completed Date: 2000-12-07 Revised Date: 2010-06-24 |
Medline Journal Info:
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Nlm Unique ID: 9434444 Medline TA: Endocrine Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 243-7 Citation Subset: IM |
Affiliation:
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Cancer Research Laboratory and Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alternative Splicing* Animals Breast / chemistry* Centrifugation, Density Gradient Epithelium / chemistry Exons Female Gene Deletion Genetic Variation* Humans Mice Mice, Nude Polymerase Chain Reaction RNA, Messenger / analysis, chemistry Receptors, Estrogen / genetics* Reverse Transcriptase Polymerase Chain Reaction Sequence Analysis, DNA |
| Grant Support | |
ID/Acronym/Agency:
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R01DK52323/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; 0/Receptors, Estrogen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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