Document Detail


Estrogen receptor {beta}1 exerts antitumoral effects on SK-OV-3 ovarian cancer cells.
MedLine Citation:
PMID:  17535880     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Estrogen receptor (ER) beta1 and its splice variants are expressed both in ovary and ovarian cancer. We studied the role of ERbeta1 and two of its splice variants in regulation of gene expression, cellular proliferation, apoptosis, and migration of an ovarian cancer cell line. In this study, we transfected SK-OV-3 ovarian cancer cells with vectors coding for ERbeta1 or its splice variants ERbeta-delta125 and ERbeta-delta1256, and tested their response to estrogen and tamoxifen in comparison with the untransfected cells. Heterologous expression of ERbeta1, but not of the exon-deleted ERbeta variants resulted in notably slower cell growth of SK-OV-3 ovarian cancer cells, an effect accompanied by more than tenfold increase of cyclin-dependent kinase inhibitor p21(WAF1) transcript levels and a significant reduction of cyclin A2 mRNA levels. SK-OV-3 cells stably overexpressing ERbeta1 ligand independently also exhibited an increased apoptosis rate and a significantly decreased motility, an effect accompanied by upregulation of fibulin 1c. Our data demonstrate that ERbeta1, but not the exon-deleted isoforms tested exerts multiple antitumoral effects on SK-OV-3 ovarian cancer cells even in the absence of estradiol or functional ERalpha.
Authors:
Oliver Treeck; Georg Pfeiler; Diana Mitter; Claus Lattrich; Gerhard Piendl; Olaf Ortmann
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  The Journal of endocrinology     Volume:  193     ISSN:  0022-0795     ISO Abbreviation:  J. Endocrinol.     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-05-30     Completed Date:  2007-08-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0375363     Medline TA:  J Endocrinol     Country:  England    
Other Details:
Languages:  eng     Pagination:  421-33     Citation Subset:  IM    
Affiliation:
Department of Obstetrics and Gynecology, Klinik für Frauenheilkunde und Geburtshilfe, University of Regensburg, Landshuter Strasse 65, 93053 Regensburg, Germany. otreeck@caritasstjosef.de
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MeSH Terms
Descriptor/Qualifier:
Alternative Splicing
Apoptosis
Blotting, Western / methods
Cell Line, Tumor
Cell Movement
Cell Proliferation
Estrogen Receptor beta / genetics,  metabolism*
Exons
Female
Gene Expression Regulation, Neoplastic*
Humans
Ovarian Neoplasms / metabolism*
Protein Isoforms / genetics,  metabolism*
RNA Interference
RNA, Small Interfering / pharmacology
Reverse Transcriptase Polymerase Chain Reaction
Transfection / methods
Wound Healing
Chemical
Reg. No./Substance:
0/Estrogen Receptor beta; 0/Protein Isoforms; 0/RNA, Small Interfering

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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