Document Detail


Estrogen receptor alpha localization in the testes of men with normal spermatogenesis.
MedLine Citation:
PMID:  23042285     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
It is known that estrogens act on the male reproductive tract by binding to estrogen receptors (ER) a andb. However, studies on ER localization in the human testis are discordant. The aim of this study was to investigatethe localization of ERa in the testes of adult men with normal spermatogenesis. Semen analysis of ten adult menrevealed azoospermia. FSH, LH and testosterone serum concentrations were within normal values, and the volumeof the testes was normal, hence obstructive azoospermia was suspected. The tissues from testicular surgicalbiopsies were fixed in Bouin's fluid and embedded in paraffin. Assessments of the seminiferous epithelium (scoring10 to -1), the number of Leydig cells (scoring 1 to 5), the areal fraction of intertubular space (IS), measurementsof seminiferous tubule diameter, and the thickness of the tubular wall, were performed on microscopicsections. Immunohistochemical staining was applied with monoclonal antibodies against ERa. The mean spermatogenesisscore was 10 points; IS - 30.6 ± 8.1%; seminiferous tubule diameter - 193.9 ± 19.4 μm; thickness oftubular wall - 7.44 ± 1.1 μm; number of Leydig cells - 1.6 ± 1.1 points. Immunohistochemical staining showedthe localization of ERa to be in the Sertoli and Leydig cell cytoplasm, while ERa was absent in germ cells. Theresults of testicular tissue analysis confirmed its normal structure and normal, full spermatogenesis. The presenceof ERa in Sertoli and Leydig cells in normal human testis demonstrated in this study suggests that estrogens mayaffect testicular function.
Authors:
Eliza Filipiak; Dagmara Suliborska; Maria Laszczynska; Renata Walczak-Jedrzejowska; Elzbieta Oszukowska; Katarzyna Marchlewska; Krzysztof Kula; Jolanta Slowikowska-Hilczer
Related Documents :
23423975 - Dynamics of senescent cell formation and retention revealed by p14arf induction in the ...
23393365 - A method to assess the distribution and frequency of plasma cells and plasma cell precu...
23440885 - Radixin expression in microglia after cortical stroke lesion.
23958055 - Enhanced mgmt expression contributes to temozolomide resistance in glioma stem-like cells.
22361945 - Investigation of basement membrane proteins in a case of granular cell ameloblastoma.
8413615 - Residues in the tata-binding protein required to mediate a transcriptional response to ...
Publication Detail:
Type:  Journal Article     Date:  2013-10-08
Journal Detail:
Title:  Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society     Volume:  50     ISSN:  1897-5631     ISO Abbreviation:  Folia Histochem. Cytobiol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-10-08     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8502651     Medline TA:  Folia Histochem Cytobiol     Country:  Poland    
Other Details:
Languages:  eng     Pagination:  340-5     Citation Subset:  IM    
Affiliation:
. jolanta.slowikowska-hilczer@umed.lodz.pl.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Trichophyton mentagrophytes-associated Majocchi's granuloma treated with cryotherapy.
Next Document:  Early gene regulation of osteogenesis in embryonic stem cells.