Document Detail

Estrogen-provided Cardiac Protection Following Burn Trauma is Mediated Through a Reduction in Mitochondria-derived DAMPs.
MedLine Citation:
PMID:  24464748     Owner:  NLM     Status:  Publisher    
Mitochondria-derived danger-associated molecular patterns (DAMPs) play important roles in sterile inflammation after acute injuries. This study was designed to test the hypothesis that 17ß-estradiol protects the heart via suppressing myocardial mitochondrial DAMPs after burn injury using an animal model. Sprague Dawley rats were given a third-degree scald burn comprising 40% total body surface area (TBSA). 17ß-estradiol, 0.5 mg/kg, or control vehicle were administered subcutaneously 15 minutes following burn. The heart was harvested 24 hours post-burn. Estradiol showed significant inhibition on the productivity of H2O2 and oxidation of lipid molecules in the mitochondria. Estradiol increased mitochondrial antioxidant defense via enhancing the activities and expression of superoxide dismutase (SOD) and glutathione peroxidase (GPx). Estradiol also protected mitochondrial respiratory function and structural integrity. In parallel, estradiol remarkably decreased burn-induced release of mitochondrial cytochrome C and mtDNA into cytoplasm. Further, estradiol inhibited myocardial apoptosis, shown by its suppression on DNA laddering and down-regulation of caspase 1 and caspase 3. Estradiol's anti-inflammatory effect was demonstrated by reduction in systemic and cardiac cytokines (TNF-α, IL-1β and IL-6), decrease in NF-κB activation, and attenuation of the expression of inflammasome component ASC in the heart of burned rats. Estradiol-provided cardiac protection was shown by reduction in myocardial injury marker troponin-I, amendment of heart morphology, and improvement of cardiac contractility after burn injury. Together, these data suggest that post-burn administration of 17ß-estradiol protects the heart via an effective control over the generation of mitochondrial DAMPs (mtROS, cytochrome C and mtDNA) that incite cardiac apoptosis and inflammation.
Xiao Yao; Jane G Wigginton; David L Maass; Lisha Ma; Deborah L Carlson; Steven E Wolf; Joseph P Minei; Qun S Zang
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-1-24
Journal Detail:
Title:  American journal of physiology. Heart and circulatory physiology     Volume:  -     ISSN:  1522-1539     ISO Abbreviation:  Am. J. Physiol. Heart Circ. Physiol.     Publication Date:  2014 Jan 
Date Detail:
Created Date:  2014-1-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100901228     Medline TA:  Am J Physiol Heart Circ Physiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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