Document Detail

Estrogen modulation of pneumonia? An immunoglobulin A effect.
MedLine Citation:
PMID:  22491603     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Laboratory and clinical studies demonstrated a salutary effect of estradiol (E2) on pneumonia and other infectious complications after trauma, while dihydrotestosterone (DHT) failed to show a similar effect. Secretory immunoglobulin A is the principle antibody in the respiratory and other mucosal secretions. Immunoglobulin A (IgA) production and transport into the mucosal secretion is regulated by Toll-like receptor 4 (TLR-4). In addition, E2 may influence immune regulatory cells via TLR-4. We hypothesized that the protective effect of E2 on the development of pneumonia may be related to modulation of IgA transport into respiratory secretions.
METHODS: Calu-3 respiratory epithelial cell monolayers were established in a two-chamber cell culture system. Calu-3 cells were then treated with either E2 or DHT for 3 days for maximal cell stimulation. Dimeric IgA was added to the basal chamber of Calu-3 cells, and IgA transcellular transport was indexed by recovery of secretory immunoglobulin A in the apical chamber media. In separate experiments, Klebsiella pneumonia (10(5) CFU/mL) was added to the apical chamber of treated Calu-3 cell monolayers, and bacterial passage across Calu-3 cells was determined by bacterial recovery from the basal chamber. Calu-3 cells not treated with E2 or DHT served as control.
RESULTS: Calu-3 cells pretreated with E2 significantly increased IgA transport, and this effect was augmented in a dose-dependent fashion. Only cells pretreated with E2 significantly decreased bacterial passage, and this effect was exhibited in a dose- and time-dependent fashion. E2 led to a significant increase in TLR-4 expression.
CONCLUSION: The protective effect of E2 against pneumonia may be related to augmented transport of IgA into the respiratory mucosal secretions.
Abubaker A Ali; Lawrence N Diebel; David M Liberati
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The journal of trauma and acute care surgery     Volume:  72     ISSN:  2163-0763     ISO Abbreviation:  J Trauma Acute Care Surg     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-04-11     Completed Date:  2012-06-27     Revised Date:  2013-09-25    
Medline Journal Info:
Nlm Unique ID:  101570622     Medline TA:  J Trauma Acute Care Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  908-15     Citation Subset:  AIM; IM    
Department of Surgery, Wayne State University, Detroit, Michigan 48201, USA.
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MeSH Terms
Cell Line
Dihydrotestosterone / pharmacology
Dose-Response Relationship, Drug
Enzyme-Linked Immunosorbent Assay
Epithelial Cells / drug effects*,  microbiology
Estradiol / pharmacology*
Immunoglobulin A / drug effects*,  metabolism,  physiology
Klebsiella Infections / drug therapy,  immunology
Klebsiella pneumoniae
Pneumonia, Bacterial / immunology*,  physiopathology
Respiratory System / cytology
Toll-Like Receptor 4 / metabolism
Reg. No./Substance:
0/Immunoglobulin A; 0/Toll-Like Receptor 4; 50-28-2/Estradiol; 521-18-6/Dihydrotestosterone
Comment In:
J Trauma Acute Care Surg. 2012 Apr;72(4):915   [PMID:  22491604 ]

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