Document Detail


Estrogen modulates neuronal movements within the developing preoptic area-anterior hypothalamus.
MedLine Citation:
PMID:  17767488     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The preoptic area-anterior hypothalamus (POA-AH) is characterized by sexually dimorphic features in a number of vertebrates and is a key region of the forebrain for regulating physiological responses and sexual behaviours. Using live-cell fluorescence video microscopy with organotypic brain slices, the current study examined sex differences in the movement characteristics of neurons expressing yellow fluorescent protein (YFP) driven by the Thy-1 promoter. Cells in slices from embryonic day 14 (E14), but not E13, mice displayed significant sex differences in their basal neuronal movement characteristics. Exposure to 10 nm estradiol-17beta (E2), but not 100 nm dihydrotestosterone, significantly altered cell movement characteristics within minutes of exposure, in a location-specific manner. E2 treatment decreased the rate of motion of cells located in the dorsal POA-AH but increased the frequency of movement in cells located more ventrally. These effects were consistent across age and sex. To further determine whether early-developing sex differences in the POA-AH depend upon gonadal steroids, we examined cell positions in mice with a disruption of the steroidogenic factor-1 gene, in which gonads do not form. An early-born cohort of cells were labelled with the mitotic indicator bromodeoxyuridine (BrdU) on E11. More cells were found in the POA-AH of females than males on the day of birth (P0) regardless of gonadal status. These results support the hypothesis that estrogen partially contributes to brain sexual dimorphism through its influence on cell movements during development. Estrogen's influence may be superimposed upon a pre-existing genetic bias.
Authors:
John Gabriel Knoll; Cory A Wolfe; Stuart A Tobet
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  The European journal of neuroscience     Volume:  26     ISSN:  0953-816X     ISO Abbreviation:  Eur. J. Neurosci.     Publication Date:  2007 Sep 
Date Detail:
Created Date:  2007-09-04     Completed Date:  2007-11-28     Revised Date:  2014-09-19    
Medline Journal Info:
Nlm Unique ID:  8918110     Medline TA:  Eur J Neurosci     Country:  France    
Other Details:
Languages:  eng     Pagination:  1091-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Age Factors
Animals
Animals, Newborn
Bromodeoxyuridine / metabolism
Cell Count / methods
Cell Movement / drug effects*,  genetics
Embryo, Mammalian
Estradiol / pharmacology*
Estrogen Receptor alpha / metabolism
Female
Hypothalamus, Anterior / cytology*,  embryology*
Luminescent Proteins / genetics,  metabolism
Male
Mice
Mice, Inbred C57BL
Mice, Transgenic
Microscopy, Video / methods
Neurons / drug effects*
Nitric Oxide Synthase Type I / metabolism
Organ Culture Techniques
Sex Factors
Steroidogenic Factor 1 / deficiency
Grant Support
ID/Acronym/Agency:
MH61376/MH/NIMH NIH HHS; R01 MH061376/MH/NIMH NIH HHS; R01 MH061376-08/MH/NIMH NIH HHS
Chemical
Reg. No./Substance:
0/Estrogen Receptor alpha; 0/Luminescent Proteins; 0/Steroidogenic Factor 1; 4TI98Z838E/Estradiol; EC 1.14.13.39/Nitric Oxide Synthase Type I; G34N38R2N1/Bromodeoxyuridine
Comments/Corrections

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