| Estrogen inhibits D2S receptor regulated Gi3 and Gs protein interactions to stimulate prolactin production and cell proliferation in lactotropic cells. | |
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MedLine Citation:
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PMID: 22573829 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The neurotransmitter dopamine is known to inhibit prolactin (PRL) secretion and the proliferation of lactotropes in the pituitary gland. Dopamine-2 (D2) receptors short (D2S) isoform is expressed in a reduced level while the D2 receptor long (D2L) isoform is expressed in an elevated level during estradiol (E2)-induced PRL production and cell proliferation in lactotropes. To evaluate the role of these D2 receptor isoforms on E2-regulated lactotropic cell function, we compared E2 effects on the level of PRL, cell proliferation and G proteins in enriched lactotropes and lactotrope-derived PR1 cells containing only D2S isoform (D2S cells), D2L isoform (D2L cells) or no D2 receptor (V cells). Additionally, we determined the effects of G protein blockade on the E2-induced PRL production and cell proliferation in these cells. We show here that E2 actions on G proteins and PRL production and cell proliferation were maximally achieved in D2S cells, oppositely or marginally achieved in D2L cells and is absent in V cells. We also show that the dopamine and pertussis toxin (PTX) modulations of E2 actions on PRL, G proteins and cell proliferation were maximally achieved in D2S cells than in D2L or V cells. Furthermore, we provide evidence for the existence of an inhibitory action of Gi3 on Gs that is under the control of the D2S receptor and is inhibited by E2. These results suggest that the suppression of D2S regulated Gi3 inhibition of Gs protein may be one of the mechanisms controlling E2-actvated PRL synthesis and cell proliferation in lactotropes. |
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Authors:
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Amitabha Sengupta; Dk K Sarkar |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-5-9 |
Journal Detail:
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Title: The Journal of endocrinology Volume: - ISSN: 1479-6805 ISO Abbreviation: - Publication Date: 2012 May |
Date Detail:
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Created Date: 2012-5-10 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0375363 Medline TA: J Endocrinol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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A Sengupta, Animal Sciences, Rutgers University, New Brunswick, United States. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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