| Estrogen-induced SDF-1 production is mediated by estrogen receptor-α in female hearts after acute ischemia and reperfusion. | |
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MedLine Citation:
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PMID: 21719062 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Gender differences exist in myocardial response to acute ischemia/reperfusion (I/R) injury and estrogen mediates cardioprotection in the female heart after I/R. Accumulating evidence has indicated that stromal cell-derived factor-1 (SDF-1) is increased in the ischemic heart and initiates cardioprotective effects. However, it is unknown whether SDF-1 plays a role in gender-specific response to myocardial I/R and in estrogen-induced acute protection. Therefore, we hypothesize that (1) increased SDF-1 production will be observed in female hearts compared with male hearts in response to I/R, which is attributable to the effect of estrogen; and that (2) estrogen receptor (ER)α, not ERβ mediates estrogen-contributed SDF-1 expression in female hearts after I/R. METHODS: Heart tissue subjected to I/R injury was assessed for myocardial expression of SDF-1 (by enzyme-linked immunosorbent assay) and SDF-1 receptor-CXCR4 (Western blot). Groups were as follows: Rat hearts from adult male, female, ovariectomized female (OVX F), and male and OVX F supplemented with chronic 17β-estradiol (E2), and mouse hearts from adult male and female wild-type, ERα knockout (ERαKO) and ERβKO. RESULTS: I/R significantly increased myocardial SDF-1 expression in both genders. Higher levels of SDF-1 existed in female hearts after I/R compared with males. Depletion of endogenous estrogen by ovariectomy reduced cardiac SDF-1 production in females after I/R. E2 supplementation significantly restored SDF-1 expression in OVX F and males compared with their counterparts. Notably, ablation of ERα, not ERβ, markedly decreased SDF-1 production in females after I/R. Unlike SDF-1, cardiac CXCR4 expression was not affected by gender, sex hormone, or ERs in the ischemic heart. CONCLUSION: Our study represents the first evidence that female hearts exhibit higher levels of SDF-1 expression compared with males after acute I/R. This increased myocardial SDF-1 production in females is partly owing to effect of estrogen through ERα, but not ERβ. |
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Authors:
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Chunyan Huang; Hongmei Gu; Yu Wang; Meijing Wang |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2011-06-30 |
Journal Detail:
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Title: Surgery Volume: 150 ISSN: 1532-7361 ISO Abbreviation: Surgery Publication Date: 2011 Aug |
Date Detail:
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Created Date: 2011-08-01 Completed Date: 2011-09-20 Revised Date: 2012-10-09 |
Medline Journal Info:
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Nlm Unique ID: 0417347 Medline TA: Surgery Country: United States |
Other Details:
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Languages: eng Pagination: 197-203 Citation Subset: AIM; IM |
Copyright Information:
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Copyright © 2011 Mosby, Inc. All rights reserved. |
Affiliation:
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Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chemokine CXCL12 / metabolism* Estrogen Receptor alpha / metabolism* Estrogens / metabolism* Female Male Mice Mice, Inbred C57BL Mice, Knockout Myocardial Reperfusion Injury / metabolism* Rats Sex Factors Up-Regulation |
| Grant Support | |
ID/Acronym/Agency:
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R00 HL087607-04/HL/NHLBI NIH HHS; R00 HL0876077/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Chemokine CXCL12; 0/Estrogen Receptor alpha; 0/Estrogens |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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