Document Detail


Estrogen-controlled gene expression in tissue culture cells by zearalenone.
MedLine Citation:
PMID:  2972561     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In two estrogen-sensitive cell lines, Le42 and MCF-7, the estrogenic potential of the nonsteroidal mycotoxin zearalenone has been investigated. The chloramphenicol acetyltransferase (CAT) gene expression in Le42 cells is induced by zearalenone after transfection with a CAT-gene construct controlled by an estrogen responsive element [(1986) Cell 46, 1053-1061]. In MCF-7 cells zearalenone induces at least 2 exoproteins (52 and 160 kDa) which are estrogen-specific [(1980) Cell 20, 353-362). These data suggest that zearalenone acts by activating the estrogen receptor. Due to the high sensitivity of these cell lines for zearalenone both test systems are proposed as assays for a quantitative estimation of the biological (estrogenic) activity of this widespread mycotoxin.
Authors:
U E Mayr
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  FEBS letters     Volume:  239     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1988 Nov 
Date Detail:
Created Date:  1988-12-20     Completed Date:  1988-12-20     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  223-6     Citation Subset:  IM    
Affiliation:
Institut für Tierernährung, Universität Hohenheim, Stuttgart, FRG.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Chloramphenicol O-Acetyltransferase / genetics
Estradiol / pharmacology*
Genes / drug effects*
Protein Biosynthesis
Receptors, Estrogen / biosynthesis
Resorcinols / pharmacology*
Transfection
Zearalenone / pharmacology*
Chemical
Reg. No./Substance:
0/Receptors, Estrogen; 0/Resorcinols; 17924-92-4/Zearalenone; 50-28-2/Estradiol; EC 2.3.1.28/Chloramphenicol O-Acetyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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