| Estrogen and aryl hydrocarbon receptor expression and crosstalk in human Ishikawa endometrial cancer cells. | |
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MedLine Citation:
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PMID: 10822009 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Ishikawa endometrial cancer cells express the estrogen receptor (ER), and this study investigates aryl hydrocarbon receptor (AhR) expression and inhibitory AhR-ER crosstalk in this cell line. Treatment of Ishikawa cells with the AhR agonist [3H]2,3,7, 8-tetrachlorodibenzo-p-dioxin (TCDD) gave a radiolabeled nuclear complex that sedimented at 6.0 S in sucrose density gradients, and Western blot analysis confirmed that Ishikawa cells expressed human AhR and AhR nuclear translocator (Arnt) proteins. Treatment of Ishikawa cells with 10 nM TCDD induced a 9.7-fold increase in CYP1A1-dependent ethoxyresorufin O-deethylase (EROD) activity and a 10.5-fold increase in chloramphenicol acetyltransferase (CAT) activity in cells transfected with pRNH11c containing an Ah-responsive human CYP1A1 gene promoter insert (-1142 to +2434). Inhibitory AhR-ER crosstalk was investigated in Ishikawa cells using E2-induced cell proliferation and transcriptional activation assays in cells transfected with E2-responsive constructs containing promoter inserts from the progesterone receptor and vitellogenin A2 genes. AhR agonists including TCDD, benzo[a]pyrene (BaP) and 6-methyl-1,3,8-trichlorodibenzofuran, inhibited 32-47% of the E2-induced responses. In contrast, neither estrogen nor progesterone inhibited EROD activity induced by TCDD in Ishikawa cells, whereas inhibitory ER-AhR crosstalk was observed in ECC-1 endometrial cells suggesting that these interactions were cell context-dependent. |
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Authors:
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M Wormke; E Castro-Rivera; I Chen; S Safe |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of steroid biochemistry and molecular biology Volume: 72 ISSN: 0960-0760 ISO Abbreviation: J. Steroid Biochem. Mol. Biol. Publication Date: 2000 Apr |
Date Detail:
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Created Date: 2000-07-06 Completed Date: 2000-07-06 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 9015483 Medline TA: J Steroid Biochem Mol Biol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 197-207 Citation Subset: IM |
Affiliation:
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Department of Veterinary Physiology and Pharmacology, Texas A & M University, TX 77843-4466, College Station, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenocarcinoma
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drug therapy,
genetics,
metabolism* Aryl Hydrocarbon Receptor Nuclear Translocator Benzo(a)pyrene / pharmacology Benzofurans / pharmacology Chloramphenicol O-Acetyltransferase / drug effects, genetics, metabolism Cytochrome P-450 CYP1A1 / drug effects, genetics, metabolism DNA-Binding Proteins* Endometrial Neoplasms / drug therapy, genetics, metabolism* Estradiol / pharmacology Female Humans Promoter Regions, Genetic Receptors, Aryl Hydrocarbon / agonists, genetics, metabolism* Receptors, Estrogen / drug effects, genetics, metabolism* Receptors, Progesterone / drug effects, genetics, metabolism Tetrachlorodibenzodioxin / pharmacology Transcription Factors / drug effects, metabolism Transcription, Genetic Tumor Cells, Cultured |
| Grant Support | |
ID/Acronym/Agency:
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CA64081/CA/NCI NIH HHS; ES09106/ES/NIEHS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/ARNT protein, human; 0/Benzofurans; 0/DNA-Binding Proteins; 0/Receptors, Aryl Hydrocarbon; 0/Receptors, Estrogen; 0/Receptors, Progesterone; 0/Transcription Factors; 115039-00-4/6-methyl-1,3,8-trichlorodibenzofuran; 138391-32-9/Aryl Hydrocarbon Receptor Nuclear Translocator; 1746-01-6/Tetrachlorodibenzodioxin; 50-28-2/Estradiol; 50-32-8/Benzo(a)pyrene; EC 1.14.14.1/Cytochrome P-450 CYP1A1; EC 2.3.1.28/Chloramphenicol O-Acetyltransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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