| Estrogen 2-hydroxylase: activity in rat tissues. | |
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MedLine Citation:
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PMID: 725979 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Incubation parameters for a radioderivative assay for estrogen 2-hydroxylase have been examined. The assay was found to be specific and sensitive if a chromatographically purified preparation of COMT was used. Estradiol was found to be a better substrate for the 2-hydroxylase than estrone or estriol. The liver had significantly higher estrogen 2-hydroxylase activity than any other tissue examined. The estrogen 2-hydroxylase was highly localized in the microsomal fraction in both the liver and the brain. The male rat was found to have significantly more estrogen 2-hydroxylase activity in the liver than the female rat. In addition, in the male rat liver, the estrogen 2-hydroxylase activity was reversibly inducible by testosterone and was not affected by phenobarbital. In the male and female rat brain the estrogen 2-hydroxylase activities were similar. |
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Authors:
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R L Barbieri; J A Canick; K J Ryan |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Steroids Volume: 32 ISSN: 0039-128X ISO Abbreviation: Steroids Publication Date: 1978 Nov |
Date Detail:
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Created Date: 1979-02-26 Completed Date: 1979-02-26 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0404536 Medline TA: Steroids Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 529-38 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brain / enzymology Castration Catechol O-Methyltransferase / metabolism Estrogens Female Kidney / enzymology Male Microsomes / drug effects, enzymology* Microsomes, Liver / enzymology Ovary / enzymology Phenobarbital / pharmacology Rats Steroid Hydroxylases / metabolism* Subcellular Fractions / enzymology Substrate Specificity Testis / enzymology Testosterone / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Estrogens; 50-06-6/Phenobarbital; 58-22-0/Testosterone; EC 1.14.-/Steroid Hydroxylases; EC 2.1.1.6/Catechol O-Methyltransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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