| Estriol enhances lipopolysaccharide-induced increases in nitric oxide production by Kupffer cells via mechanisms dependent on endotoxin. | |
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MedLine Citation:
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PMID: 12198378 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Estriol causes sensitization of Kupffer cells to lipopolysaccharide (LPS) via mechanisms dependent on gut-derived LPS. Accordingly, this study examines the effect of estriol treatment on nitric oxide (NO) production from Kupffer cells. METHODS: Rats were given estriol (20 mg/kg body weight) intraperitoneally, and Kupffer cells were isolated 24 hr later. Some rats were treated for 4 days with 150 mg/kg/day of polymyxin B and 450 mg/kg/day of neomycin to prevent growth of intestinal bacteria, the primary source of endotoxin in the gastrointestinal tract. After addition of LPS, NO production by Kupffer cell was detected using a fluorescence indicator, DAF-2. RESULTS: Twenty-four hours after estriol administration, LPS-induced NO production by Kupffer cells was enhanced as compared with control Kupffer cells. Sterilization of the gut with antibiotics blocked this enhancement. CONCLUSIONS: Estriol treatment in vivo enhances LPS-induced NO production in Kupffer cells. |
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Authors:
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Nobuyuki Enomoto; Yoshiyuki Takei; Tsuneo Kitamura; Miyoko Hirose; Kenichi Ikejima; Nobuhiro Sato |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Alcoholism, clinical and experimental research Volume: 26 ISSN: 0145-6008 ISO Abbreviation: Alcohol. Clin. Exp. Res. Publication Date: 2002 Aug |
Date Detail:
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Created Date: 2002-08-28 Completed Date: 2003-03-07 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7707242 Medline TA: Alcohol Clin Exp Res Country: United States |
Other Details:
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Languages: eng Pagination: 66S-69S Citation Subset: IM |
Affiliation:
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Department of Gastroenterology, Juntendo University School of Medicine, Tokyo, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bacteria / drug effects, immunology Estriol / pharmacology* Female Intestines / microbiology Kupffer Cells / drug effects*, immunology Lipopolysaccharides / immunology* Microscopy, Fluorescence Neomycin / pharmacology Nitric Oxide / metabolism* Polymyxin B / pharmacology Rats Rats, Wistar |
| Chemical | |
Reg. No./Substance:
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0/Lipopolysaccharides; 10102-43-9/Nitric Oxide; 1404-04-2/Neomycin; 1404-26-8/Polymyxin B; 50-27-1/Estriol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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