Document Detail


Estradiol-stimulated turnover of heparan sulfate proteoglycan in mouse uterine epithelium.
MedLine Citation:
PMID:  2965148     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Heparan sulfate proteoglycans (HSPGs) and dermatan sulfate/chondroitin sulfate proteoglycans may be extracted from the uterine epithelium of immature mice by a 1-min exposure of the luminal surface of excised uteri to 1% Nonidet P-40 detergent. In mice that are treated with estradiol there is a marked increase in free heparan sulfate glycosaminoglycan in the extract. (a) By Sepharose exclusion chromatography the [35S]sulfate-labeled major HSPG had a nominal Mr of 200-250 X 10(3), consisting of a core protein of about 80-90 X 10(3) Mr with about 8-10 heparan sulfate glycosaminoglycan chains (Mr = 13 X 10(3)). The HSPG had a lower bouyant density (less than 1.45 g/ml) than the dermatan sulfate/chondroitin sulfate proteoglycan and was heterogeneous, as was evident in the fact that HSPG attained equilibrium over a wide range of CsCl densities and also showed nonuniform interaction with octyl-Sepharose. (b) Virtually all of the major HSPG was removed when the epithelium was isolated by proteolysis, indicating a cell surface localization. A smaller, less prominent HSPG (nominal Mr = 80 X 10(3)) was synthesized during the first 2 h after isolation. (c) Label and chase experiments with and without chloroquine showed that virtually all of the free heparan sulfate glycosaminoglycan chains derived from endocytosis and lysosomal degradation of the plasma membrane-associated HSPG. We conclude that estradiol stimulates endocytosis of HSPG, predominantly from the basolateral epithelial surface and suggest that this HSPG turnover may reflect changes associated with blastocyst attachment and invasion of the endometrium.
Authors:
J E Morris; S W Potter; G Gaza-Bulseco
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  263     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1988 Apr 
Date Detail:
Created Date:  1988-05-02     Completed Date:  1988-05-02     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4712-8     Citation Subset:  IM    
Affiliation:
Department of Zoology, Oregon State University, Corvallis 97331.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cells, Cultured
Epithelium / drug effects,  metabolism
Estradiol / pharmacology*
Female
Glycosaminoglycans / metabolism*
Heparan Sulfate Proteoglycans
Heparitin Sulfate / isolation & purification,  metabolism*
Kinetics
Mice
Pregnancy
Proteochondroitin Sulfates / isolation & purification,  metabolism*
Proteoglycans / isolation & purification,  metabolism*
Uterus / metabolism*
Grant Support
ID/Acronym/Agency:
HD-19530/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Glycosaminoglycans; 0/Heparan Sulfate Proteoglycans; 0/Proteochondroitin Sulfates; 0/Proteoglycans; 50-28-2/Estradiol; 9050-30-0/Heparitin Sulfate

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