Document Detail


Estradiol fatty acid esters. The isolation and identification of the lipoidal derivative of estradiol synthesized in the bovine uterus.
MedLine Citation:
PMID:  7068614     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The bovine uterus, like other estrogen-responsive organs of man and rat, synthesizes an unusual nonpolar metabolite of estradiol (Schatz, F., and Hochberg, R. B. (1981) Endocrinology 109, 697-703). This compound, the lipoidal derivative of estradiol (LE2), was synthesized from estradiol by bovine endometrial tissue in vitro, and it was purified by extensive chromatography on two adsorption columns, a reversed phase partition celite column and three different high pressure liquid chromatography columns. LE2 was separated into nine fractions which were analyzed by direct probe mass spectroscopy and by gas chromatography mass spectroscopy after cleavage of the lipoidal moieties. In this manner, 10 fatty acid esters of estradiol, exclusively esterified at C-17 of the steroid nucleus, were identified. The unsaturated fatty acid esters of estradiol comprise more than 85% of the total LE2 and estradiol 17 beta-arachidonate is the most abundant component. The cholesterol esters and phospholipids of the bovine endometrium were also purified and it was found that the distribution of fatty acids in these lipids is far different from that of LE2.
Authors:
S H Mellon-Nussbaum; L Ponticorvo; F Schatz; R B Hochberg
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  257     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1982 May 
Date Detail:
Created Date:  1982-06-24     Completed Date:  1982-06-24     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  5678-84     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle
Chromatography, High Pressure Liquid
Estradiol / analogs & derivatives*,  metabolism
Fatty Acids / analysis,  metabolism*
Female
Gas Chromatography-Mass Spectrometry
Mass Spectrometry
Uterus / metabolism*
Grant Support
ID/Acronym/Agency:
AM-07328/AM/NIADDK NIH HHS; CA-29591/CA/NCI NIH HHS; HD-05077/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Fatty Acids; 50-28-2/Estradiol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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