Document Detail


Estradiol fatty acid esterification is increased in high density lipoprotein subclass 3 isolated from hypertriglyceridemic subjects.
MedLine Citation:
PMID:  16111685     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Estrogen fatty acid esters are potent lipophilic estrogens transported exclusively in lipoproteins. They are formed in HDL in a reaction catalyzed by LCAT which is considered a prerequisite for their antioxidative action. Our previous studies in normotriglyceridemic (NTG) individuals demonstrated that estradiol (E2) esterification occurred mainly in HDL3 causing accumulation of esterified, but not of unesterified E2 in the lipoprotein particles. Using HDL obtained from hypertriglyceridemic (HTG) patients, we now investigated the effect of altered HDL composition on E2 esterification. Ultracentrifugally isolated HDL2 and HDL3 from NTG- and HTG-males were incubated in an in vitro model system with radioactive and with supraphysiological concentrations of non-radioactive E2 with and without exogenous LCAT. After purification, copper-induced oxidation of HDL was measured by monitoring conjugated diene formation. The results demonstrated that (i) E2 esterification occurring mainly in HDL3 was significantly more efficient in HTG-HDL3 compared to NTG-HDL3, (ii) triglyceride content in HDL3 correlated positively with E2 esterification rate, and (iii) addition of both exogenous LCAT and E2 into the incubation prolonged lag time of HDL3 oxidation. Thus, HDL composition regulates LCAT-facilitated E2 esterification but the in vivo role of this finding can be verified only in experiments using physiological hormone concentrations.
Authors:
Anna Höckerstedt; Matti Jauhiainen; Matti J Tikkanen
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-08-18
Journal Detail:
Title:  Atherosclerosis     Volume:  185     ISSN:  0021-9150     ISO Abbreviation:  Atherosclerosis     Publication Date:  2006 Apr 
Date Detail:
Created Date:  2006-03-06     Completed Date:  2006-08-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0242543     Medline TA:  Atherosclerosis     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  264-70     Citation Subset:  IM    
Affiliation:
Department of Medicine, Division of Cardiology, Helsinki University Central Hospital, FIN-00290 Helsinki, Finland. anna.hockerstedt@hus.fi
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MeSH Terms
Descriptor/Qualifier:
Esterification
Estradiol / metabolism*
Fatty Acids / metabolism*
Humans
Hypertriglyceridemia / blood,  metabolism*
Lipoproteins, HDL / blood*
Lipoproteins, HDL3
Male
Oxidation-Reduction
Phosphatidylcholine-Sterol O-Acyltransferase / metabolism
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Lipoproteins, HDL; 0/Lipoproteins, HDL3; 50-28-2/Estradiol; EC 2.3.1.43/Phosphatidylcholine-Sterol O-Acyltransferase

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