Document Detail

Estimations of BCR-ABL/ABL transcripts by quantitative PCR in chronic myeloid leukaemia after allogeneic bone marrow transplantation and donor lymphocyte infusion.
MedLine Citation:
PMID:  11755463     Owner:  NLM     Status:  MEDLINE    
Serial assays of qualitative (multiplex and nested) and quantitative PCR were carried out for detecting and estimating the level of BCR-ABL transcripts in 39 CML patients following bone marrow transplantation. Seven of these patients, who received donor lymphocyte infusions (DLIs) following to relapse, were also monitored. Quantitative estimates of BCR-ABL transcripts were obtained by co-amplification with a competitor sequence. Estimates of ABL transcripts were used, an internal control and the ratio BCR-ABL/ABL was thus estimated for evaluating the kinetics of residual clones. Twenty four patients were followed shortly after BMT; two of these patients were in cytogenetic relapse coexisting with very high BCR-ABL levels while other 22 were in clinical, haematologic and cytogenetic remission 2-42 months after BMT. In this latter group, seven patients showed a favourable clinical-haematological progression in association with molecular remission while in 14 patients quantitative PCR assays indicated molecular relapse that was not associated with an early cytogenetic-haematologic relapse. BCR-ABL/ABL levels could not be correlated with presence of GVHD in 24 patients after BMT. In all seven patients treated with DLI, high levels of transcripts were detected at least 4 months before the appearance of clinical haematological relapse. Following DLI, five of these patients showed decreasing transcript levels from 2 to 5 logs between 4 and 12 months. In eight other patients studied long after BMT, five showed molecular relapse up to 117 months post-BMT and only one showed cytogenetic relapse. Our findings indicated that quantitative estimates of BCR-ABL transcripts were valuable for monitoring minimal residual disease in each patient.
Ivone B Otazú; Rita de Cassia B Tavares; Rocío Hassan; Ilana Zalcberg; Daniel G Tabak; Héctor N Seuánez
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Leukemia research     Volume:  26     ISSN:  0145-2126     ISO Abbreviation:  Leuk. Res.     Publication Date:  2002 Feb 
Date Detail:
Created Date:  2001-12-28     Completed Date:  2002-03-27     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7706787     Medline TA:  Leuk Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  129-41     Citation Subset:  IM    
Division of Genetics, Instituto Nacional de Câncer, Praça da Cruz Vermelha, 23, 20230-130, Rio de Janeiro, Brazil.
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MeSH Terms
Antineoplastic Agents / therapeutic use
Bone Marrow Transplantation*
Combined Modality Therapy
Disease-Free Survival
Follow-Up Studies
Fusion Proteins, bcr-abl / biosynthesis,  genetics*
Graft Enhancement, Immunologic
Leukemia, Myelogenous, Chronic, BCR-ABL Positive / drug therapy,  genetics,  metabolism*,  pathology,  therapy
Lymphocyte Transfusion*
Middle Aged
Neoplasm, Residual
Polymerase Chain Reaction / methods*
Predictive Value of Tests
RNA, Messenger / analysis*,  biosynthesis
RNA, Neoplasm / analysis*,  biosynthesis
Remission Induction
Sensitivity and Specificity
Transplantation, Homologous
Tumor Markers, Biological / biosynthesis,  genetics*
Reg. No./Substance:
0/Antineoplastic Agents; 0/Fusion Proteins, bcr-abl; 0/RNA, Messenger; 0/RNA, Neoplasm; 0/Tumor Markers, Biological

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