Document Detail


Estimation of ursodeoxycholic acid in human and bear biles using Clostridium absonum 7 beta-hydroxysteroid dehydrogenase.
MedLine Citation:
PMID:  6581749     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ursodeoxycholic acid was estimated in bile samples from humans and wild North American black bears using 7 beta-hydroxysteroid dehydrogenase purified from Clostridium absonum by Procion Red affinity chromatography. The percentage ursodeoxycholic acid was calculated by two methods: (a) 7 beta-hydroxyl groups were quantified using 7 beta-hydroxysteroid dehydrogenase and 3 alpha-hydroxyl groups (total bile acids) were quantified using 3 alpha-hydroxysteroid dehydrogenase. The percentage ursodeoxycholic acid was calculated on the basis of [7 beta-hydroxyl groups]/[3 alpha-hydroxyl groups] X 100. (b) Bile was hydrolyzed with sodium hydroxide and subjected to thin-layer chromatography. Bands corresponding to cholic acid, chenodeoxycholic acid plus deoxycholic acid, and ursodeoxycholic acid were identified by the use of standards and Komarowsky's spray reagent. Total bile acids and total ursodeoxycholic acid were measured by elution of silica gel in unsprayed areas corresponding to the bile acid standards and quantification of the total bile acid in each eluate. Direct comparison of these methods validated the use of 7 beta-hydroxysteroid dehydrogenase in the estimation of ursodeoxycholic acid in the biles of black bears and of patients fed ursodeoxycholic acid for cholesterol gallstone dissolution. Relative percentages of ursodeoxycholic acid were 8-24% in four bears and 22 and 27% in the patients ingesting 500 and 750 mg ursodeoxycholic acid per day for 3 months, respectively. Predictably lower values were obtained in two control subjects and one patient ingesting 750 mg chenodeoxycholic acid per day for 3 months.
Authors:
I A MacDonald; C N Williams; J D Sutherland; A C MacDonald
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical biochemistry     Volume:  135     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  1983 Dec 
Date Detail:
Created Date:  1984-02-24     Completed Date:  1984-02-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  349-54     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Bile / analysis*
Chenodeoxycholic Acid / therapeutic use
Chromatography, Thin Layer
Clostridium / enzymology*
Deoxycholic Acid / analogs & derivatives*
Humans
Hydroxysteroid Dehydrogenases / analysis*
Spectrophotometry
Ursidae
Ursodeoxycholic Acid / analysis*,  therapeutic use
Chemical
Reg. No./Substance:
128-13-2/Ursodeoxycholic Acid; 474-25-9/Chenodeoxycholic Acid; 83-44-3/Deoxycholic Acid; EC 1.1.-/Hydroxysteroid Dehydrogenases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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