| Estimation of treatment time for microbial preprocessing of biomass. | |
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MedLine Citation:
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PMID: 20703957 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Biochemical conversion of lignocellulosic biomass to ethanol involves size reduction, preprocessing, pretreatment, enzyme hydrolysis, and fermentation. In recent years, microbial preprocessing has been gaining attention as a means to produce labile biomass for lessening the requirement of pretreatment severity. However, loss of sugars due to microbial consumption is a major consequence, suggesting its minimization through optimization of nutrients, temperature, and preprocessing time. In this work, we emphasized estimation of fungal preprocessing time, at which higher sugar yields can be achieved after preprocessing and enzyme hydrolysis. The estimation is based on the enzymatic activity profile obtained by treating switchgrass with Phanerochaete chrysosporium for 28 days. Enzyme assays were conducted once in every 7 days for 28 days, for activities of phenol oxidase, peroxidase, beta-glucosidase, beta-xylosidase, and cellobiohydrolase. We found no activity for phenol oxidase and peroxidase, but the greatest activities for cellulases on the seventh day. We then treated switchgrass for 7 days with P. chrysosporium and observed that the preprocessed switchgrass had higher glucan (39%), xylan (17.5%), and total sugar yields (25.5%) than the unpreprocessed switchgrass (34%, 37.5%, and 20.5%, respectively, p < 0.05). This verifies the utility of using enzyme assays for initial estimation of preprocessing time to enhance sugar yields. |
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Authors:
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Swetha Mahalaxmi; Colin R Jackson; Clint Williford; Charles L Burandt |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2010-02-21 |
Journal Detail:
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Title: Applied biochemistry and biotechnology Volume: 162 ISSN: 1559-0291 ISO Abbreviation: Appl. Biochem. Biotechnol. Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-08-12 Completed Date: 2010-11-22 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8208561 Medline TA: Appl Biochem Biotechnol Country: United States |
Other Details:
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Languages: eng Pagination: 1414-22 Citation Subset: IM |
Affiliation:
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Department of Chemical Engineering, University of Mississippi, University, MS 38655, USA. swetha.mahalaxmi@gmail.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Biomass* Biotechnology / methods* Glucose / analysis Phanerochaete / enzymology, metabolism* Poaceae / metabolism Time Factors |
| Chemical | |
Reg. No./Substance:
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50-99-7/Glucose |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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