| Estimation of trans-resveratrol in herbal extracts and dosage forms by high-performance thin-layer chromatography. | |
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MedLine Citation:
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PMID: 15930785 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A simple, sensitive and precise high-performance thin-layer chromatographic (HPTLC) method of analysis of trans-resveratrol in Polygonum cuspidatum root extracts and in dosage forms was developed and validated. The separation was carried out on a TLC aluminium plates precoated with silica gel 60F-254 as the stationary phase, eluted with chloroform-ethylacetate-formic acid (2.5 : 1 : 0.1) as mobile phase. Densitometric analysis of trans-resveratrol was carried out in the absorbance mode at 313 nm. This system was found to give compact spot for trans-resveratrol (Rf value of 0.40+/-0.03). A good linear regression relationship between peak areas and the concentrations was obtained over the range of 0.5-3.0 microg/spot with correlation coefficient 0.9989. The limit of detection and quantification was found to be 9 and 27 ng/spot. The method was validated for precision and recovery. The spike recoveries were within 99.85 to 100.70%. The RSD values of the precision in the range 0.37-1.84%. The proposed developed HPTLC method can be applied for identification and quantitative determination of trans-resveratrol in herbal extracts and dosage forms. |
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Authors:
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Sukala Kanna Babu; Katta Vijaya Kumar; Gottumukkala Venkata Subbaraju |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Chemical & pharmaceutical bulletin Volume: 53 ISSN: 0009-2363 ISO Abbreviation: Chem. Pharm. Bull. Publication Date: 2005 Jun |
Date Detail:
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Created Date: 2005-06-02 Completed Date: 2006-01-09 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0377775 Medline TA: Chem Pharm Bull (Tokyo) Country: Japan |
Other Details:
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Languages: eng Pagination: 691-3 Citation Subset: IM |
Affiliation:
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Laila Impex Research Centre, Unit I, Phase III, Jawahar Autonagar, Vijayawada-520 007, India. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chromatography, High Pressure Liquid
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methods Chromatography, Thin Layer / methods Dosage Forms Plant Extracts / administration & dosage, analysis Plant Preparations / administration & dosage*, analysis* Statistics as Topic Stilbenes / administration & dosage*, analysis* |
| Chemical | |
Reg. No./Substance:
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0/Dosage Forms; 0/Plant Extracts; 0/Plant Preparations; 0/Stilbenes; 501-36-0/resveratrol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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