Document Detail

Estimation of the physical distance between major genomic markers in the Werner syndrome locus (8p11.2-12) by dual-color fish analysis.
MedLine Citation:
PMID:  8996964     Owner:  NLM     Status:  MEDLINE    
The gene responsible for Werner syndrome (WS) is considered to be located between D8S131 and D8S87 in the 8p11.2-12 region that includes the closest marker D8S339 (Goto et al. (1992) Nature 355: 735-738). In this experiment, the order of major markers in this region was determined and the physical distances between them were estimated by dual-color fluorescence in situ hybridization (FISH) using P1, PAC and cosmid clone DNAs as probes. The fine overall order of telomere-D8S131-D8S339-GSR-PP2A beta-D8S283-D8S87-centromere was determined for the first time. The distance from D8S131 to D8S87 was estimated to be 1,634 kb. To our surprise, the distance between D8S131 and D8S87 is much shorter than previously estimated by recombination analysis, 8.3 cM equivalent to 8.3 Mb in physical distance. These information provide the basis for the positional cloning of WS gene and the identification of its mutation.
Y Tokutake; M Satoh; N Suzuki; M Sugimoto; M Sugawara; M Goto; Y Furuichi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Japanese journal of human genetics     Volume:  41     ISSN:  0916-8478     ISO Abbreviation:  Jpn. J. Hum. Genet.     Publication Date:  1996 Sep 
Date Detail:
Created Date:  1997-02-26     Completed Date:  1997-02-26     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  9213239     Medline TA:  Jpn J Hum Genet     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  291-7     Citation Subset:  IM    
AGENE Research Institute, Kamakura, Japan.
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MeSH Terms
DNA / chemistry
Genetic Markers*
In Situ Hybridization, Fluorescence
Nucleic Acid Conformation
Werner Syndrome / genetics*
Reg. No./Substance:
0/Genetic Markers; 9007-49-2/DNA

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