Document Detail


Estimation of isotonic point of incubation medium for two-cell mouse embryo.
MedLine Citation:
PMID:  22803061     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Osmolarity of Dulbecco's medium at which the volume of two-cell mouse embryo remained similar to that of intact embryo was determined. The method is based on comparison of kinetic curves describing the volume of embryonic cell in solutions of different osmolarity. The blastomere volume was measured by quantitative laser microtomography after fixed osmotic stress intervals. It was found that Dulbecco's saline with 125 mM NaCl solution is an isotonic solution for two-cell mouse embryo. This concentration corresponds to 290 mOsm, which is lower than osmolarity (app. 310 mOsm) of media routinely used for culturing of differentiated cells or biological fluids, e.g. blood plasma.
Authors:
M A Pogorelova; V A Golichenkov; V N Pogorelova; E V Kornienko; A I Panait; A G Pogorelov
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Bulletin of experimental biology and medicine     Volume:  152     ISSN:  1573-8221     ISO Abbreviation:  Bull. Exp. Biol. Med.     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2012-07-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372557     Medline TA:  Bull Exp Biol Med     Country:  United States    
Other Details:
Languages:  eng; rus     Pagination:  142-5     Citation Subset:  IM    
Affiliation:
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino; Biological Faculty, M. V. Lomonosov Moscow State University; Pushchino State University, Russia. agpogorelov@rambler.ru.
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