| Estimation of carvedilol in human plasma by using HPLC-fluorescence detector and its application to pharmacokinetic study. | |
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MedLine Citation:
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PMID: 17702675 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A simple, precise and sensitive high performance liquid chromatography procedure has been developed for determination of carvedilol in human plasma. The method was developed on Lichrosphere R CN column using a mobile phase of acetonitrile/20 mM ammonium acetate buffer with 0.1% triethylamine (pH adjusted to 4.5) (40/60, v/v). The peaks were detected by using fluorescence detector (excitation wavelength 282 nm and emission wavelength 340 nm). Carvedilol and domperidone (internal standard) were extracted by liquid-liquid extraction procedure using dichloromethane. This method was specific and had a linearity range of 1-128 ng/ml with intra- and inter-day precision (%C.V.) less than 15%. The accuracy ranges from 87.3 to 100.88% and the recovery of carvedilol was 69.90%. The stability studies showed that carvedilol in human plasma was stable during short-term period for sample preparation and analysis. This method was used to assay the carvedilol in human plasma samples obtained from subjects who had been given an oral tablet of 12.5 mg carvedilol. |
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Authors:
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Rajeshwari Rathod; L Poorna Chandra Prasad; Shubha Rani; Manish Nivsarkar; Harish Padh |
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Publication Detail:
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Type: Clinical Trial; Journal Article Date: 2007-07-22 |
Journal Detail:
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Title: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Volume: 857 ISSN: 1570-0232 ISO Abbreviation: J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. Publication Date: 2007 Oct |
Date Detail:
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Created Date: 2007-09-28 Completed Date: 2007-12-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101139554 Medline TA: J Chromatogr B Analyt Technol Biomed Life Sci Country: Netherlands |
Other Details:
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Languages: eng Pagination: 219-23 Citation Subset: IM |
Affiliation:
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B.V. Patel Pharmaceutical Education and Research Development (PERD) Centre, Thaltej-Ghandhinagar Highway, Thaltej, Ahmedabad 380 054, India. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Administration, Oral Adrenergic beta-Antagonists / administration & dosage, blood*, chemistry, pharmacokinetics* Adult Calibration Carbazoles / administration & dosage, blood*, chemistry, pharmacokinetics* Chromatography, High Pressure Liquid / instrumentation* Drug Stability Fluorescence Humans Propanolamines / administration & dosage, blood*, chemistry, pharmacokinetics* Quality Control Reference Standards |
| Chemical | |
Reg. No./Substance:
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0/Adrenergic beta-Antagonists; 0/Carbazoles; 0/Propanolamines; 72956-09-3/carvedilol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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