Document Detail


Estimation of the biological activity (potency) of batches of brucellin prepared from a mucoid strain of Brucella abortus.
MedLine Citation:
PMID:  9791877     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A study was conducted to determine the repeatability of a procedure used to prepare brucellin from a mucoid strain of Brucella abortus, and to determine the biological activity of those brucellins. The brucellins were standardized to contain 1 mg protein/ml, and their potency was estimated according to the European pharmacopoeia norm for tuberculin. Estimation of the potency was done in cattle that have been sensitized with living or killed brucellae. A brucellin that effectively detected acute and chronic brucellosis in cattle with experimentally induced brucellosis was used as reference brucellin. Results show that five of the nine batches of brucellin equalled the potency of the reference brucellin. Three brucellins had 79-88% potency of the reference brucellin and one had only 59%. Since the potency of various brucellins may vary it is suggested to estimate the potency of the brucellins according to the European pharmacopoeia norm for tuberculin.
Authors:
Z Bercovich; G J Tetenburg; A Eger
Related Documents :
25424157 - Home-range allometry in coral reef fishes: comparison to other vertebrates, methodologi...
17821007 - Schistosoma mansoni: natural infection of cattle in brazil.
16068177 - An independent microbial flora of the epithelium and its role in the ecomicrobiology of...
23576617 - Identification of hypothalamic neuron-derived neurotrophic factor as a novel factor mod...
18377527 - The design and deployment of the helex septal occluder.
15499217 - Role of dietary intake and intestinal absorption of oxalate in calcium stone formation.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Veterinary microbiology     Volume:  62     ISSN:  0378-1135     ISO Abbreviation:  Vet. Microbiol.     Publication Date:  1998 Aug 
Date Detail:
Created Date:  1998-12-02     Completed Date:  1998-12-02     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7705469     Medline TA:  Vet Microbiol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  313-20     Citation Subset:  IM    
Affiliation:
Department of Bacteriology, Institute for Animal Science and Health (ID-DLO), Lelystad, The Netherlands. z.bercovich@id.dlo.nl
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Allergens
Animals
Antigens, Bacterial / immunology*,  isolation & purification,  pharmacology
Brucella abortus / immunology*
Cattle
Europe
Immunization
Pharmacopoeias as Topic
Skin / immunology
Skin Tests / veterinary
Chemical
Reg. No./Substance:
0/Allergens; 0/Antigens, Bacterial; 11028-17-4/brucellin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Protective effects of equine herpesvirus-1 (EHV-1) glycoprotein B in a murine model of EHV-1-induced...
Next Document:  Preliminary characterisation of extracellular serine proteases of Dermatophilus congolensis isolates...