Document Detail


Estimation of aqueous distributional spaces in the dual perfused rat liver.
MedLine Citation:
PMID:  11018118     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
1. The aim of this study was to estimate the aqueous distributional spaces of the liver as a function of the route of input: portal vein (PV) versus hepatic artery (HA). 2. Studies were performed in the situ single (PV) and dual (PV-HA) perfused rat liver (n = 6-10) using Krebs bicarbonate buffer at constant PV (12 ml min-1) and HA (3 ml min-1) flow rates. An impulse input-output response technique was employed, varying the route of input, using non-labelled erythrocytes (intravascular marker), 125I-albumin and [14C]sucrose (extracellular markers), and [14C]urea and 3H2O (total water markers) as the reference indicators. 3. Distributional spaces were estimated using two different methods, namely standard and specific. The standard method was applied to hepatic outflow data obtained from the single PV perfused liver. The specific method was used when operating in the dual perfused mode to provide an estimate of the excess space perfused solely by the HA input. Specific spaces, interstitial and intracellular volumes, were estimated by difference. 4. The results were evaluated by means of visual inspection of the outflow profiles and comparison of the distributional spaces. Different hepatic effluent profiles obtained as a function of the route of input indicated that these two inputs did not completely mix within the liver. Estimates of the distributional spaces supported this observation, and further suggested that the arterial input perfuses 9-12 % more hepatic tissue than the venous input. 5. The knowledge obtained from the existence of a specific arterial space can be extended to help make predictions about the fate of an eliminated compound following arterial administration. Any difference between the HA and PV in terms of hepatic recovery could be attributed to this excess space and its enzyme density.
Authors:
S Sahin; M Rowland
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of physiology     Volume:  528 Pt 1     ISSN:  0022-3751     ISO Abbreviation:  J. Physiol. (Lond.)     Publication Date:  2000 Oct 
Date Detail:
Created Date:  2000-11-14     Completed Date:  2000-11-30     Revised Date:  2013-06-11    
Medline Journal Info:
Nlm Unique ID:  0266262     Medline TA:  J Physiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  199-207     Citation Subset:  IM    
Affiliation:
Faculty of Pharmacy, University of Hacettepe, 06100-Ankara, Turkey and School of Pharmacy and Pharmaceutical Sciences, University of Manchester, Manchester M13 9PL, UK.
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MeSH Terms
Descriptor/Qualifier:
Animals
Body Fluid Compartments / physiology*
Erythrocyte Transfusion
Erythrocytes / metabolism
Hepatic Artery / physiology
Injections, Intra-Arterial
Injections, Intravenous
Liver / blood supply,  metabolism*
Male
Perfusion
Portal Vein / physiology
Rats
Rats, Sprague-Dawley
Serum Albumin / administration & dosage,  metabolism
Sucrose / administration & dosage,  metabolism
Urea / administration & dosage,  metabolism
Water / administration & dosage,  metabolism*
Chemical
Reg. No./Substance:
0/Serum Albumin; 57-13-6/Urea; 57-50-1/Sucrose; 7732-18-5/Water
Comments/Corrections

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