Document Detail


Estimation of the stability of parathyroid hormone when stored at -80 degrees C for a long period.
MedLine Citation:
PMID:  19820128     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND AND OBJECTIVES: Stability of parathyroid hormone (PTH) at -80 degrees C for long storage periods has never been studied. This can be of importance for the conclusions of studies where blood banks have been constituted. The study's aim was to evaluate stability of PTH when stored as serum or plasma EDTA samples at -80 degrees C.
DESIGN, SETTING, PARTICIPANTS & MEASUREMENTS: Samples were collected from 16 chronic hemodialysis patients using EDTA and gel-separator tubes. Plasma and serum were aliquoted; one aliquot was assayed with Elecsys and Liaison methods to determine the "baseline" values and another aliquot after 1, 3, 6, and 12 mo. The factors "method," "tubes," "subjects," and "time" were included in a mixed linear model to evaluate their effects on measured PTH values. The prediction interval methodology was used to assess where a future result could be obtained with a defined probability.
RESULTS: With the Liaison method, the maximum storage times with either dry or EDTA tubes were estimated to be 9 and 2 mo, respectively. With the Elecsys method, samples could be stored at least 2 yr with acceptable level of degradation.
CONCLUSION: PTH stability at -80 degrees C is not infinite. Maximum storage time and acceptance limits (30%) were defined, showing that with one method, samples should be stored for not more than 2 mo, whereas the other could be stored for up to 2 yr. With any PTH assay, the maximum storage time should be evaluated to ascertain that samples will keep their initial reactive profile after prolonged storage periods.
Authors:
Etienne Cavalier; Pierre Delanaye; Philippe Hubert; Jean-Marie Krzesinski; Jean-Paul Chapelle; Eric Rozet
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Validation Studies     Date:  2009-10-09
Journal Detail:
Title:  Clinical journal of the American Society of Nephrology : CJASN     Volume:  4     ISSN:  1555-905X     ISO Abbreviation:  Clin J Am Soc Nephrol     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-12-09     Completed Date:  2010-03-01     Revised Date:  2013-05-31    
Medline Journal Info:
Nlm Unique ID:  101271570     Medline TA:  Clin J Am Soc Nephrol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1988-92     Citation Subset:  IM    
Affiliation:
Department of Clinical Chemistry, University of Liège, Domaine du Sart-Tilman, Liège, Belgium. Etienne.cavalier@chu.ulg.ac.be
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MeSH Terms
Descriptor/Qualifier:
Anticoagulants
Blood Banks / standards*
Chemistry, Clinical / standards*
Cold Temperature
Cryopreservation / standards*
Edetic Acid
Humans
Kidney Failure, Chronic / blood,  therapy
Models, Biological
Parathyroid Hormone / analysis*,  blood
Renal Dialysis
Reproducibility of Results
Specimen Handling
Chemical
Reg. No./Substance:
0/Anticoagulants; 0/Parathyroid Hormone; 60-00-4/Edetic Acid
Comments/Corrections

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