Document Detail


Estimation of LDL-associated apolipoprotein B from measurements of triglycerides and total apolipoprotein B.
MedLine Citation:
PMID:  18443176     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: VLDL and chylomicrons may interfere with measurements of apolipoprotein B (apo B) on LDL particles. Ultracentrifugation of samples enriched in chylomicrons and VLDL and subsequent measurement of apo B in the infranate fraction [density (d) = 1.006] removes this interference. This apo B fraction is called "LDL-apo B." METHODS: We retrospectively analyzed 64 895 measurements of triglycerides, total apo B, and LDL-apo B. Samples were ultracentrifuged, and 3 commercially available immunoassays that use different antibodies were used to measure LDL-apo B in the 1.006 infranate fraction. RESULTS: After adjusting for triglyceride concentration, we found total apo B and LDL-apo B measurements to be strongly correlated. We derived a simple linear equation for calculating LDL-apo B concentration (in milligrams per deciliter) from measurements of total apo B and triglycerides: LDL-apo B = apo B - 10 mg/dL - triglycerides/32. This equation accurately predicts LDL-apo B values within +/- 12% of the measured value in 75% of cases. CONCLUSIONS: Our equation provides a convenient means of estimating LDL-apo B from commonly available measurements of total apo B and triglycerides without the need for ultracentrifugation. LDL-apo B measurements were also independent of the different apo B antibodies in the 3 assays used in this study. An equation that predicts LDL-apo B particle number may be useful, regardless of the apo B assay used.
Authors:
Arthur M Baca; G Russell Warnick
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinical chemistry     Volume:  54     ISSN:  0009-9147     ISO Abbreviation:  Clin. Chem.     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-04-29     Completed Date:  2008-06-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  907-10     Citation Subset:  IM    
Affiliation:
Berkeley Heartlab Inc, Alameda, CA 94501, USA. abaca@bhlinc.com
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MeSH Terms
Descriptor/Qualifier:
Algorithms
Apolipoproteins B / blood*
Humans
Immunoassay
Lipoproteins, LDL / blood*
Retrospective Studies
Triglycerides / blood*
Ultracentrifugation
Chemical
Reg. No./Substance:
0/Apolipoproteins B; 0/Lipoproteins, LDL; 0/Triglycerides
Comments/Corrections
Comment In:
Clin Chem. 2008 May;54(5):782-4   [PMID:  18443175 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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