Document Detail


Estimation of glucose uptake by ovarian follicular cells.
MedLine Citation:
PMID:  21769539     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In vitro maturation (IVM) of mammalian oocytes provides an alternative to traditional in vitro fertilization techniques for clinical treatment of infertility or animal breeding. IVM involves the collection of oocytes from the ovary prior to ovulation, with maturation occurring in a laboratory environment. The success of IVM is highly sensitive to the in vitro nutrient environment. The nurse cells surrounding the oocyte, known as cumulus cells, regulate this environment and removal of these cells reduces the ability of the oocyte to develop following insemination. Determining the nature of the interaction between the oocyte and cumulus cells, collectively called the cumulus-oocyte complex (COC), is a difficult task experimentally. Here we use a combination of experimental and mathematical techniques to investigate glucose transport within bovine COCs and find quantitative estimates of the glucose uptake rates of the oocyte and cumulus cells. Surprisingly, our modeling shows the rate of uptake of glucose by the oocyte to increase and then decrease with concentration, a result that needs further experimental investigation but which supports the expectation that high and low glucose concentrations are detrimental to oocyte development. The methodology described is suitable for use across species and for investigating the transport of other important nutrients within the COC.
Authors:
A R Clark; Y M Stokes; J G Thompson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-07-16
Journal Detail:
Title:  Annals of biomedical engineering     Volume:  39     ISSN:  1573-9686     ISO Abbreviation:  Ann Biomed Eng     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-09-16     Completed Date:  2012-03-16     Revised Date:  2013-05-30    
Medline Journal Info:
Nlm Unique ID:  0361512     Medline TA:  Ann Biomed Eng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2654-67     Citation Subset:  IM    
Affiliation:
Auckland Bioengineering Institute, The University of Auckland, Auckland, New Zealand. alys.clark@auckland.ac.nz
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MeSH Terms
Descriptor/Qualifier:
Algorithms
Animals
Biological Transport
Cattle
Cells, Cultured
Coculture Techniques
Computer Simulation*
Cumulus Cells / physiology
Female
Fertilization in Vitro
Glucose / metabolism*
Humans
Models, Biological
Oocytes* / growth & development,  metabolism
Oogenesis / physiology*
Chemical
Reg. No./Substance:
50-99-7/Glucose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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