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Estimation of Free, Conjugated, and Diffusible Indole-3-acetic Acid in Etiolated Maize Shoots by the Indolo-alpha-pyrone Fluorescence Method.
MedLine Citation:
PMID:  16662325     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
Procedures for estimating free indoleacetic acid (IAA extracted from tissue homogenates by aqueous acetone), conjugated IAA (extracted by aqueous acetone and hydrolyzed by 1 n KOH), and diffusible IAA (diffused from the excised tissue into water), in shoots of etiolated 3-day-old maize (Zea mays L. cv. GH 390) seedlings are described, the indolo-alpha-pyrone fluorescence method being used to assay IAA. The reliability of the procedure is shown by comparative IAA determinations of the extracts using the gas chromatography-mass spectrometry method in which the methyl ester, heptafluorobutyryl derivative of IAA is assayed using the selected-ion-monitoring technique with deuterated IAA as an internal standard. A 3-millimeter-long coleoptile tip, a coleoptile with its included leaves and nodal region (whole coleoptile), and a mesocotyl each contains 0.2, 1.7, and 1.5 nanograms of free IAA, respectively. The whole coleoptile and the mesocotyl contain slightly less conjugated IAA than their content of free IAA. IAA diffuses from the coleoptile tip at the rate of 1.0 nanograms per tip per hour; from the base of the whole coleoptile and a set of leaves excised from a coleoptile, IAA diffuses at the rate of 0.62 and 0.17 nanogram per plant part per hour, respectively. The data obtained support the classical assumption that the coleoptile tip produces IAA. It is also suggested that some IAA is decomposed during its downward transport in the coleoptile.
Authors:
M Iino; D J Carr
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Plant physiology     Volume:  69     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  1982 Apr 
Date Detail:
Created Date:  2010-06-29     Completed Date:  2010-06-29     Revised Date:  2010-09-14    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  950-6     Citation Subset:  -    
Affiliation:
Department of Developmental Biology, Research School of Biological Sciences, The Australian National University, Canberra City, Australian Capital Territory 2601, Australia.
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